PPB023

Tris buffer

Pre-set, pH 8.1, pHast Pack^™, powder

• 别名: 0.1M Tris
• 分類程式碼代碼: 12161700
• NACRES: NA.25

属性

• 品質等級: 100
• 產品線: pHast Pack^™
• 形狀: powder
• 儲存條件: (Tightly closed. Dry)
• 雜質: ≤5 ppm Heavy Metals (lead)
• 顏色: white
• pH值: 8.1
• 正離子痕跡: Fe: ≤10 ppm
• 異物活動: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 儲存溫度: room temp

说明

一般說明

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

應用

Intended for use in:

• Cell culture (sterile filter recommended)
• Western blot
• Protein electrophoresis
• DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

特點和優勢

• Ready-to-use for quick buffer prep
• No measuring and adjusting pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包裝

Foil pouches

重構

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

儲存和穩定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他說明

For additional information on our range of Biochemicals, please complete this form.

法律資訊

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1