10874515001

苯丁抑制素 盐酸盐

95% (HPLC), crystalline powder, suitable for protein purification

• 别名: N-\ [(2S,3R)-3-氨基-2-羟基-4-苯基丁酰]- L -亮氨酸 盐酸盐
• 经验公式（希尔记法）: C₁₆H₂₄N₂O₄ · HCl
• CAS号: 65391-42-6
• 分子量: 344.83
• Beilstein: 4628066
• MDL號碼: MFCD00058004
• 分類程式碼代碼: 12352204
• PubChem物質ID: 329749214

属性

• 描述: [(2S,3R)-3-Amino-2-hydroxy-4-phenylbutanoyl]-L-leucine hydrochloride
• 化驗: 95% (HPLC)
• 形狀: crystalline powder
• 包裝: pkg of 10 mg
• 製造商／商標名: Roche
• 儲存條件: protect from light
• 技術: protein purification: suitable
• mp: 195-205 °C
• 溶解度: water: soluble 25 g/L; alcohol: soluble 5 g/L
• λmax: 255-260 nm at 1 N in hydrochloric acid
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• SMILES 字串: O=C(N[C@@H](CC(C)C)C(O)=O)[C@@H](O)[C@H](N)CC1=CC=CC=C1.Cl[H]
• InChI: 1S/C16H24N2O4.ClH/c1-10(2)8-13(16(21)22)18-15(20)14(19)12(17)9-11-6-4-3-5-7-11;/h3-7,10,12-14,19H,8-9,17H2,1-2H3,(H,18,20)(H,21,22);1H/t12-,13+,14+;/m1./s1
• InChI 密鑰: XGDFITZJGKUSDK-UDYGKFQRSA-N

说明

應用

Bestatin has been used as a protease inhibitor for the purification of his-tagged Tau protein.

生化／生理作用

一种氨肽酶选择性金属蛋白酶抑制剂。苯丁抑制素是亮氨酸氨基肽酶、氨基肽酶 B 和三氨基肽酶的竞争性和特异性抑制剂。在 60 nM 抑制氨肽酶 B（用精氨酸-β-萘胺为底物）和亮氨酸氨基肽酶在 20 nM（亮氨酸-β-萘胺为底物）。其显示对氨肽酶 A、胰蛋白酶、糜蛋白酶、弹性蛋白酶、木瓜蛋白酶、胃蛋白酶或溶血素无抑制作用。它有希望成为一种新型镇痛药，因为它能保护内源性阿片肽免受降解。

Bestatin exhibits anti-angiogenic property and inhibits new blood vessel formation at the early stage of tumor growth and in the metastatic sites.

準備報告

Working concentration: 40 μg/ml (130 μM)
Working solution: Recommended solvents are 1 M HCl, methanol or 0.15 M NaCl.
Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl.
For best results, use a stock solution of 2–5 mg/ml in methanol.
Storage conditions (working solution): -15 to -25 °C
Avoid repeated freezing!

重構

Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl. Roche recommends preparing a stock solution of 2 to 5 mg/ml in methanol.
Solutions are stable for 6 months when stored in aliquots at -15 to -25 °C.

儲存和穩定性

Store at 2 to 8 °C. (Store dry!)

其他說明

仅用于生命科学研究。不可用于诊断。

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable