推荐产品
生物源
mouse
品質等級
抗體表格
unpurified
抗體產品種類
primary antibodies
無性繁殖
FK2, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性（以同源性預測）
all
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG1
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
可识别K29-、K48-和K63-连接的多泛素化和单泛素化蛋白，但不能识别Raji细胞中的游离泛素。
抗泛素小鼠mAb（FK2）经过验证可用于ELISA、免疫印迹、免疫沉淀和免疫荧光法，以检测泛素。
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与骨髓瘤细胞融合而产生的小鼠单克隆抗体。可识别K29-、K48-和K63-连接的多泛素化和单泛素化蛋白。
免疫原
人
未纯化的多聚泛素化溶菌酶
應用
ELISA（参见应用参考文献）
免疫印迹法（1:1000）
免疫沉淀法（参见应用参考文献和备注）
免疫荧光法（见应用参考文献）
包裝
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外觀
溶于PBS中。
準備報告
Raji细胞
重構
初次融化后，等分并冷冻（-20°C）。稀释抗体可在4°C下储存最长1个月。
其他說明
Matsumoto, M., et al. 2005.Proteomics 5, 4545.
Boname, J.N., et al. 2001.Immunity 15, 627.
Campbell, V., et al. 2001.Neuron 32, 1013.
Fujimuro, M., et al. 2003.FEBS Letts.349, 173.
Boname, J.N., et al. 2001.Immunity 15, 627.
Campbell, V., et al. 2001.Neuron 32, 1013.
Fujimuro, M., et al. 2003.FEBS Letts.349, 173.
不能识别游离泛素。对于免疫沉淀，一旦抗体被固定，它就显示出对游离泛素以及多泛素化和单泛素化蛋白的亲和力。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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