324699

Elastase Substrate IV, Colorimetric

A specific substrate for pancreatic elastase.

• 别名: Elastase Substrate IV, Colorimetric
• 经验公式（希尔记法）: C₂₅H₃₄N₆O₉
• 分子量: 562.57
• 分類程式碼代碼: 12352200
• NACRES: NA.54

属性

• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: lyophilized solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles; desiccated
• 顏色: white to off-white
• 溶解度: ethanol: 1 mg/mL; water: soluble
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

A specific substrate for pancreatic elastase (K_m = 100 µM; K_cat/K_m = 35,000 s^-1 M^-1 for rat pancreatic elastase; K_m = 30 µM; K_cat/K_m = 351,000 s^-1 M^-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol: see Largman, C. (1983)

A specific substrate for pancreatic elastase (k_m = 100 µM; k_cat/ K_m = 35,000 M^-1 s^-1 for rat pancreatic elastase, K_m = 30 µM; k_cat/K_m = 35,000 M^-1 s^-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.

生化／生理作用

Cell permeable: no

Primary Target
A specific substrate for pancreatic elastase

Product does not compete with ATP.

Reversible: no

km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine

警告

Toxicity: Standard Handling (A)

序列

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

外觀

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

重構

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

其他說明

Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable