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Merck
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主要文件

06-1328

Sigma-Aldrich

Anti-Themis Antibody

from rabbit, purified by affinity chromatography

别名:

thymocyte selection associated, thymocyte selection pathway associated, chromosome 6 open reading frame 190, chromosome 6 open reading frame 207, protein THEMIS, Thymocyte-expressed molecule involved in selection

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

100

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

mouse

种属反应性（根据同源性预测）

rat (73% sequence homology)

技术

flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

NP_848781

UniProt登记号

Q8BGW0

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

mouse ... Themis(210757)

相关类别

一般描述

Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells.

特异性

This antibody recognizes Themis at the C-terminus.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis.

应用

Detect Themis using this Anti-Themis Antibody validated for use in WB, FC.

质量

Evaluated by Western Blot in mouse thymocyte tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.

目标描述

~72 kDa observed.
UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa

分析说明

Control
Mouse thymocyte tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Mukul Prasad et al.
Cellular & molecular immunology, 18(9), 2249-2261 (2020-11-13)
Themis is a T cell lineage-specific molecule that is involved in TCR signal transduction. The effects of germline Themis deletion on peripheral CD4+ T cell function have not been described before. In this study, we found that Themis-deficient CD4+ T
Differential function of Themis CABIT domains during T cell development.
Okada, T; Nitta, T; Kaji, K; Takashima, A; Oda, H; Tamehiro, N; Goto, M; Okamura et al.
Testing null
Namrata Gautam et al.
Life science alliance, 6(12) (2023-09-23)
Themis is important in regulating positive selection of thymocytes during T cell development, but its role in peripheral T cells is less understood. Here, we investigated T cell activation and its sequelae using a tamoxifen-mediated, acute Themis deletion mouse model.
Themis sets the signal threshold for positive and negative selection in T-cell development.
Fu, G; Casas, J; Rigaud, S; Rybakin, V; Lambolez, F; Brzostek, J; Hoerter, JA; Paster et al.
Nature null
Joanna Brzostek et al.
Nature immunology, 21(2), 186-198 (2020-01-15)
T cell homeostasis and functional responsiveness require signals from self-peptide-major histocompatibility complex (self-pMHC) and cytokines, but the mechanisms controlling this signal integration are unknown. Using a conditional deletion of the T cell lineage-specific protein Themis, we show that Themis is

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