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Merck

W4125

Sigma-Aldrich

Williams′ Medium E

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Medium E, Williams E Medium, Williams’ E medium

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L
glucose: 2.0 g/L
phenol red: 0.0107 g/L
NaHCO3: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Descripción general

Williams′ E medium is a rich medium developed by Williams′ and Gunn. It is recommended for supporting long-term adult liver epithelial cell culture.

Aplicación

Williams′ E medium has been used in culturing mice/rat primary hepatocytes.

Cantidad

Formulated to contain 10.8 grams of powder per liter of medium.

Reconstitución

Supplement with 2.2 g/L sodium bicarbonate.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Etienne Guillocheau et al.
The Journal of nutritional biochemistry, 63, 19-26 (2018-10-14)
Trans-palmitoleic acid (trans-C16:1 n-7 or trans-Δ9-C16:1, TPA) is believed to improve several metabolic parameters according to epidemiological data. TPA may mainly come from direct intakes: however, data are inconsistent due to its very low amount in foods. Instead, TPA might
M Herranz-Itúrbide et al.
Redox biology, 40, 101841-101841 (2021-01-26)
Liver is a unique organ in displaying a reparative and regenerative response after acute/chronic damage or partial hepatectomy, when all the cell types must proliferate to re-establish the liver mass. The NADPH oxidase NOX4 mediates Transforming Growth Factor-beta (TGF-β) actions
Christina P Keravnou et al.
Ultrasound in medicine & biology, 42(11), 2676-2686 (2016-08-25)
Localized drug delivery and uptake can benefit from the combined action of ultrasound and microbubbles at a specific site. Some of the possible mechanisms suggested are vessel poration and/or cell poration, but the exact acoustic parameters that trigger those phenomena
Xue Yu et al.
Acta biochimica et biophysica Sinica, 41(12), 1027-1032 (2009-12-17)
Homocysteine is an intermediate in the sulfur amino acid metabolism. Recent studies suggested that there might be links between hyperhomocysteinemia and insulin resistance. In the present study, we investigated the effect of homocysteine on glucose metabolism. We demonstrated that the
D M Toivola et al.
Journal of cell science, 110 ( Pt 1), 23-33 (1997-01-01)
The importance of protein phosphatases in the maintenance of cytoskeletal structure is supported by the serious liver injury caused by microcystin-LR, a hepatotoxic inhibitor of type-1 and type-2A serine/threonine protein phosphatases. We used the microcystin-LR-induced cell injury as a model

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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