The stability of stock solutions of this enzyme has not been evaluated. However, it is cited in Methods in Enzymology, 165, 3-7 (1988) that at concentrations greater than 2 mg of protein per ml it can be stored at -20 °C without loss of hemolytic titer.
H9395
α-Hemolysin from Staphylococcus aureus
lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein
Sinónimos:
α-Toxin
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432,00 €
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1150,00 €
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0.5 MG
432,00 €
1 MG
1150,00 €
About This Item
432,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Solicitar un pedido a granel
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origen biológico
Staphylococcus aureus
Nivel de calidad
Formulario
lyophilized powder
actividad específica
≥10,000 units/mg protein
contiene
sodium citrate buffer as balance
composición
Protein, ~60% Lowry
solubilidad
H2O: soluble 0.49-0.51 mg/mL
Nº de acceso UniProt
temp. de almacenamiento
2-8°C
Información sobre el gen
Staphylococcus aureus ... SAOUHSC_01121(3920722)
Descripción general
Aplicación
α-Hemolysin from Staphylococcus aureus has been used:
- as a component of electrolyte solution for testing pore formation in lipid bilayer using electrophysiological measurements[2]
- to test its osteogenesis suppressive effects in bone marrow stromal cells (BMSCs)[3]
- in the preparation of α-hemolysin molecular imprinted polymer (MIP) for Biacore and surface plasmon resonance[4]
α-Hemolysin was used in a study to test the efflux pump and haemolysin activity of Escherichia coli of dairy origin. It was also used to test its adaptation to benzalkonium chloride and the effect of ciprofloxacin on biofilm formation.[5]
Acciones bioquímicas o fisiológicas
α-Hemolysin is selectively hemolytic and the monomeric form binds to a membrane and specific receptors are not required for binding. Upon binding to biological membranes and/or artificial membranes, self-oligomerization occurs, resulting in ring structures (hexameric aggregates) believed to represent transmembrane pores, which are permeable to ions and small metabolites.[1] It has a marked preference for rabbit red blood cells.[6] α-hemolysin stimulates cellular phospholipases and induces a Ca2+ influx.[7] It leads to membrane disruption of the endothelial barrier and leakage of cytoplasmic components[1] and osmotic lysis of the cells.[6] α-hemolysin is implicated in the pathogenesis of sepsis.[4]
Envase
Package size based on protein content
Definición de unidad
One hemolytic unit will cause 50% lysis of a 1% suspension of rabbit red blood cells in phosphate buffered saline, pH 7.0, containing 1% bovine serum albumin after 30 min at 37 °C followed by refrigeration for 30 min at 4 °C.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2
Órganos de actuación
Lungs,Blood
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Gregory J Digby et al.
The Journal of physiology, 586(14), 3325-3335 (2008-05-24)
Signalling by heterotrimeric G proteins is often isoform-specific, meaning certain effectors are regulated exclusively by one family of heterotrimers. For example, in excitable cells inwardly rectifying potassium (GIRK) channels are activated by G betagamma dimers derived specifically from G(i/o) heterotrimers.
Ankita Pagedar et al.
The Journal of dairy research, 79(4), 383-389 (2012-08-10)
The present study investigates the effect of adaptive resistance to ciprofloxacin (Cip) and benzalkonium chloride (BC) on biofilm formation potential (BFP), efflux pump activity (EPA) and haemolysin activity of Escherichia coli isolates of dairy origin. All the isolates, irrespective of
François Vandenesch et al.
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One key aspect of the virulence of Staphylococcus aureus lies in its ability to target the host cell membrane with a large number of membrane-damaging toxins and peptides. In this review, we describe the hemolysins, the bi-component leukocidins (which include
D Fink et al.
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Staphylococcal alpha-toxin at subcytotoxic concentrations stimulated phosphatidylinositol turnover and arachidonic acid release in undifferentiated cultures of pheochromocytoma PC12 cells. Stimulation of phospholipase A2 but not C was dependent on extracellular calcium. Addition of staphylococcal alpha-toxin to PC12 cells caused a
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Phosphoinositides (PtdIns) play important roles in exocytosis and are thought to regulate secretory granule docking by co-clustering with the SNARE protein syntaxin to form a docking receptor in the plasma membrane. Here we tested this idea by high-resolution total internal
Contenido relacionado
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Los métodos de lisis celular y extracción de proteínas abarcan varias técnicas, desde la solubilización de detergentes hasta la ruptura mecánica, que respaldan las necesidades de investigación.
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We suspended in the product H9395 in 12.5 ml of PBS (our usual water-based infection solvent), flash-froze the aliquots in liquid nitrogen, and stored the aliquots at -80 C. There was no apparent issue with resuspension in PBS vs water. However, we observed no hemolytic activity in our in vivo mouse study or an in vitro quantitative hemolysis assay performed with rabbit blood.
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