G2751
Glutamic-Oxalacetic Transaminase from porcine heart
Type I, ammonium sulfate suspension, 200-500 units/mg protein
Sinónimos:
L-Aspartate:2-oxoglutarate aminotransferase, Aspartate Aminotransferase, GOT
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1000 UNITS
162,00 €
2000 UNITS
303,00 €
10000 UNITS
1190,00 €
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1000 UNITS
162,00 €
2000 UNITS
303,00 €
10000 UNITS
1190,00 €
About This Item
162,00 €
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Type I
Nivel de calidad
Formulario
ammonium sulfate suspension
actividad específica
200-500 units/mg protein
actividad extraña
glutamic-pyruvic transaminase ≤0.03%
lactic dehydrogenase ≤0.01%
malic dehydrogenase ≤0.01%
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
Research area: Cellsignaling. Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle.[1] It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.[2]
Aplicación
Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.
Glutamic-Oxalacetic Transaminase from porcine heart has been used:
- in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity[3]
- in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples[4]
- as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system[5]
Acciones bioquímicas o fisiológicas
Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate.[6] Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.[7]
Definición de unidad
One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.
Forma física
Suspension in 3.0 M (NH4)2SO4 containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0
Nota de análisis
Protein determined by biuret.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Artículos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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