F1757

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

• Sinónimos: L-Malate hydrolyase, Fumarate hydratase
• Número CAS: 9032-88-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-887-7
• MDL number: MFCD00131111
• Número CE: 4.2.1.2 (BRENDA, IUBMB)
• Specific activity: ≥300 units/mg protein (biuret)

Propiedades

• form: ammonium sulfate suspension
• Quality Segment: 200
• specific activity: ≥300 units/mg protein (biuret)
• foreign activity: malic dehydrogenase ≤0.1%
• storage temp.: 2-8°C

Descripción

General description

Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Application

Fumarase from porcine heart has been used:

• as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
• for immobilized enzyme based stability assay
• in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

Biochem/physiol Actions

Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 0.05 M KH₂PO₄, pH 7.5, 0.014 M 2-mercaptoethanol

Preparation Note

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

Other Notes

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

Información de seguridad

• Pictogramas: GHS07
• Palabra de advertencia: Warning
• Códigos de peligro: H317
• Indicaciones de precaución: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable