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Merck

E6412

Sigma-Aldrich

Cellobiohydrolase I from Hypocrea jecorina

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0.13 U/mg, recombinant, expressed in corn

Sinónimos:

Cel7A, Cellobiosidase, Cellulase

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100 UNITS
161,00 €

161,00 €


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100 UNITS
161,00 €

About This Item

Comisión internacional de enzimas:
Número CE:
Código UNSPSC:
12352204
NACRES:
NA.54

161,00 €


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recombinante

expressed in corn

Nivel de calidad

Formulario

liquid

actividad específica

0.13 U/mg

características de los productos alternativos más sostenibles

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sustainability

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categoría alternativa más sostenible

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Cellubiohydrolase I is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 53 kDa with a catalytic domain and a cellulose binding domain. The reaction adds water to the glucose bonds in cellulose (non-reducing ends of the chain), yielding cellobiose.
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Aplicación

Cellobiohydrolase I can be used in combination with endocellulases and b-glucosidase to produce glucose from cellulose.

Acciones bioquímicas o fisiológicas

Cellobiohydrolase (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. CBH I cleaves progressively from the reducing end. CBH I is commonly used in detergents for cleaning textiles.[1] Its ezymatic activity ranges from 37° C to 50° C, with its optimal temperature being approximately 45° C. The optimum pH for the enzyme is 5-6.

Definición de unidad

Unit Definition: A unit will turn over 1 nmole of methyl-umbelliferyl beta-D cellobioside per min at pH 5 at 50° C.

Forma física

Provided as an ammonium sulfate precipitate with the source as recombinant maize.

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Kiyohiko Igarashi et al.
Methods in enzymology, 510, 169-182 (2012-05-23)
Cellulases hydrolyze β-1,4-glucosidic linkages of insoluble cellulose at the solid/liquid interface, generating soluble cellooligosaccharides. We describe here our method for real-time observation of the behavior of cellulase molecules on the substrate, using high-speed atomic force microscopy (HS-AFM). When glycoside hydrolase
Larissa C Textor et al.
The FEBS journal, 280(1), 56-69 (2012-11-02)
Aiming to contribute toward the characterization of new, biotechnologically relevant cellulolytic enzymes, we report here the first crystal structure of the catalytic core domain of Cel7A (cellobiohydrolase I) from the filamentous fungus Trichoderma harzianum IOC 3844. Our structural studies and
Svein J Horn et al.
Methods in enzymology, 510, 69-95 (2012-05-23)
Natural cellulolytic enzyme systems as well as leading commercial cellulase cocktails are dominated by enzymes that degrade cellulose chains in a processive manner. Despite the abundance of processivity among natural cellulases, the molecular basis as well as the biotechnological implications
Naohisa Sugimoto et al.
Langmuir : the ACS journal of surfaces and colloids, 28(40), 14323-14329 (2012-09-07)
Cellobiohydrolases (CBHs) hydrolyzing crystalline cellulose share a two-domain structure of catalytic domain (CD) and cellulose-binding domain (CBD). To focus on the binding characteristics of CBD, we analyzed the adsorption of fusion protein of fungal family 1 CBD from Trichoderma reesei
Microbial cellulases-Production, applications and challenges.
Sukumaran RK, et al.
J. Sci. Ind. Res., 64(11), 832-844 (2005)

Artículos

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Preguntas

  1. Good morning. This material is liquid. Could you tell me the volume that comes in the bottle?

    1 respuesta
    1. This product is provided as a slurry containing 10 mg of enzyme in saturated ammonium sulfate precipitate. It is supplied at 100 units, with a concentration of 0.13 units per 1 mg of protein at 10 mg per ml.

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