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Millipore

CELLULYSIN® Cellulase, Trichoderma viride

Sinónimos:

β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.54

origen biológico

fungus (Trichoderma viride)

formulario

solid

actividad específica

≥10,000 units/g dry wt.

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

técnicas

transformation: suitable

color

yellow-white

solubilidad

water: soluble

idoneidad

suitable for molecular biology

aplicaciones

food and beverages
pharmaceutical

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Descripción general

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.

Aplicación

CELLULYSIN® Cellulase, Trichoderma viride has been used:
  • to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
  • in the enzyme mix used for in situ hybridization to plant chromosomes
  • in the enzyme solution for the transformation of Phytophthora species
  • to isolate intact cells and protoplasts from plant tissues

Acciones bioquímicas o fisiológicas

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.

Advertencia

Toxicity: Harmful (C)

Definición de unidad

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Otras notas

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Weizhen Wang et al.
Frontiers in microbiology, 10, 2402-2402 (2019-11-12)
Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection markers for Phytophthora transformation, however
Farjana Islam et al.
BMC research notes, 11(1), 445-445 (2018-07-06)
This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC)
In Situ Hybridization to Plant Chromosomes
Fluorescence In Situ Hybridization (FISH), 447-494 null
Man-Wah Li et al.
PloS one, 10(8), e0135033-e0135033 (2015-08-14)
Arabidopsis High Mobility Group Box (HMBG) proteins were previously found associated with the interphase chromatin but not the metaphase chromosome. However, these studies are usually based on immunolocalization analysis involving paraformaldehyde fixation. Paraformaldehyde fixation has been widely adapted to preserved
Rahman Ebrahimzadegan et al.
Scientific reports, 9(1), 19989-19989 (2019-12-29)
Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing

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