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Merck

G4511

Sigma-Aldrich

β-Glucosidase from almonds

greener alternative

lyophilized powder, 10-50 units/mg solid

Sinónimos:

β-D-Glucoside glucohydrolase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

formulario

lyophilized powder

Nivel de calidad

actividad específica

10-50 units/mg solid

mol peso

50-75 kDa

características de los productos alternativos más sostenibles

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

impurezas

salt, essentially free

categoría alternativa más sostenible

temp. de almacenamiento

2-8°C

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Descripción general

β-Glucosidase from almonds belongs to the family 1 of the glycoside hydrolases. Almonds contain prunasin hydrolases (PH) and amygdalin hydrolase. Nine types of PHs are associated with almonds. PHs have signal peptide sequence and correspond to molecular weight in the range 50 to 75 kDa. PHs also have ITENG, NEP and INKKGIEYY motifs conserved and have N-glycosylation sites.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Aplicación

β-Glucosidase from almonds has been used:
  • in enzyme inhibition studies by 1,5-dideoxy-1,5-imino-d-xylitol(DIX) derivates
  • as a control in for comparison of soybean isofavone glycosides degradation by β-Glucosidase from Talaromyce leycettanus
  • as a medium component during saccharification and fermentation of yeast

β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.

Acciones bioquímicas o fisiológicas

Prunasin hydrolase (PH) is a β-glucosidase that degrades prunasin to mandelonitrile and glucose. Amygdalin hydrolase hydrolyzes amygdalin to prunasin and glucose. β-Glucosidase is mesostable and is sensitive to temperature at 60 °C and above. It is highly sensitive to high pressure.
βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.

Definición de unidad

One unit will liberate 1.0 μmole of glucose from salicin per min at pH 5.0 at 37 °C.

Nota de preparación

Chromatographically purified

inhibidor

Referencia del producto
Descripción
Precios

sustrato

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificados de análisis (COA)

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The stability of almond beta-glucosidase during combined high pressure-thermal processing: a kinetic study
Terefe N, et al.
Applied Microbiology and Biotechnology, 97(7), 2917-2928 (2013)
Matthias Lechtenberg et al.
Planta medica, 88(13), 1209-1222 (2021-11-17)
A systematic survey of Aralia spinosa (Araliaceae), covering an entire growing season and including aboveground organs at various developmental stages, revealed that only about half of all samples collected showed cyanogenesis. Cyanogenesis was detected in inflorescences and leaves but is
Galaihalage K S Ananda et al.
Planta, 255(2), 51-51 (2022-01-28)
Australian native species of sorghum contain negligible amounts of dhurrin in their leaves and the cyanogenesis process is regulated differently under water-stress in comparison to domesticated sorghum species. Cyanogenesis in forage sorghum is a major concern in agriculture as the
M N Sohail et al.
Planta, 255(4), 74-74 (2022-03-01)
Droughted sorghum had higher concentrations of ROS in both wildtype and dhurrin-lacking mutants. Dhurrin increased in wildtype genotypes with drought. Dhurrin does not appear to mitigate oxidative stress in sorghum. Sorghum bicolor is tolerant of high temperatures and prolonged droughts.
J E Busch et al.
Microbiology (Reading, England), 143(6), 2021-2026 (1997-06-01)
Thermophilic actinomycetes establish themselves as numerically dominant bacterial populations in selected high temperature environments by virtue of their exoenzymic ability to degrade the complex polysaccharides in thermogenic plant biomass. When Thermomonospora curvata and Thermomonospora fusca were grown on a mixture

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