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D9692

Plant Protoplast Digest/Wash Solution

Rapid isolation of viable protoplasts from plant tissue

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Tamaño de envaseSKUDisponibilidadPrecio
1 L
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187,00 €

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NACRES:
NA.56
UNSPSC Code:
41105500

187,00 €


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Quality Segment

form

liquid

storage temp.

2-8°C

General description

The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin. The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.

Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression,[1] viral transfection assays,[2] somatic hybridization,[3] electrophysiological studies, and morphological studies.[4]

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Este artículo
D5688PE0230G2N350
storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

-

form

-


Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Anna Kärkönen et al.
Methods in molecular biology (Clifton, N.J.), 715, 1-20 (2011-01-12)
Plant tissue cultures are an efficient system to study cell wall biosynthesis in living cells in vivo. Tissue cultures also provide cells and culture medium where enzymes and cell wall polymers can easily be separated for further studies. Tissue cultures
Shiu-Cheung Lung et al.
Plant cell reports, 30(4), 473-484 (2010-11-26)
Although transient gene expression using reporters such as green fluorescent protein is a versatile tool for examining gene functions and intracellular protein trafficking, the establishment of a highly efficient gene manipulation method remains a challenge in many plant species. A
Advanced patch-clamp techniques and single-channel analysis.
White, P. J. et al
The Journal of Experimental Biology, 50, 1037-1054 (1999)



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SKUGTIN
D9692-1L04061833591017

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