D9692
Plant Protoplast Digest/Wash Solution
Rapid isolation of viable protoplasts from plant tissue
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1 L
180,00 €
180,00 €
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1 L
180,00 €
About This Item
Código UNSPSC:
41105500
NACRES:
NA.56
180,00 €
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Descripción general
The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin.[1] The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression,[2] viral transfection assays,[3] somatic hybridization,[4] electrophysiological studies,[5] and morphological studies.[6]
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression,[2] viral transfection assays,[3] somatic hybridization,[4] electrophysiological studies,[5] and morphological studies.[6]
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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The cell wall.
Carpita, N., and McCann, M. et al.
Biochemistry, 52-108 null
Vera Bandmann et al.
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To analyze the kinetics and size of single exo- and endocytotic events in BY-2 protoplasts, we employed cell-attached membrane capacitance measurements. These measurements revealed different modes of fusion and fission of single vesicles. In about half of the observed exocytotic
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