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P4716

Sigma-Aldrich

Pectinase from Aspergillus niger

greener alternative

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Sinónimos:

Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

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About This Item

Número de CAS:
9032-75-1
Comisión internacional de enzimas:
3.2.1.15 (BRENDA, IUBMB)
Número CE:
232-885-6
Número MDL:
MFCD00131809
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

Aspergillus niger

Línea del producto

BioReagent

formulario

aqueous glycerol solution

actividad específica

≥5 units/mg protein (Lowry)

características de los productos alternativos más sostenibles

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnicas

cell culture | plant: suitable

categoría alternativa más sostenible

, Enabling

temp. de almacenamiento

2-8°C

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Descripción general

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Aplicación

Petctinase from Aspergillus niger has been used:
  • for protoplasts liberation
  • in the protoplast enzyme mixture for enzyme digestion
  • to conduct partial saccharification of sugars
  • to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Acciones bioquímicas o fisiológicas

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.

Definición de unidad

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Forma física

Solution in 40% glycerol

Otras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Karyotypes of some species of Castanopsis, Lithocarpus and Quercus (Fagaceae) from Khun Mae Kuang Forest in Chiang Mai province, northern Thailand
Chokchaichamnankit P, et al.
Thai Forest Bulletin , 38-44 (2007)
Tony Heitkam et al.
The Plant journal : for cell and molecular biology, 103(1), 32-52 (2020-01-26)
If two related plant species hybridize, their genomes may be combined and duplicated within a single nucleus, thereby forming an allotetraploid. How the emerging plant balances two co-evolved genomes is still a matter of ongoing research. Here, we focus on
Tolerance mechanisms in mercury-exposed Chromolaena odorata (lf) RM King et H. Robinson, a potential phytoremediator
Alcantara HJP, et al.
Journal of Degraded and Mining Lands Management, 1(1), 09-20 (2013)
Trude Schwarzacher
Methods in molecular biology (Clifton, N.J.), 1370, 87-103 (2015-12-15)
Good preparations are essential for informative analysis of both somatic and meiotic chromosomes, cytogenetics, and cell divisions. Fluorescent chromosome staining allows even small chromosomes to be visualized and counted, showing their morphology. Aneuploidies and polyploidies can be established for species
Paulina Tomaszewska et al.
Annals of botany (2021-12-08)
Diploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa are important for food security and the environment​, often being planted in marginal lands worldwide. We aimed to characterize the nature of their
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Protocolos

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

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