This product is not tested for the presence of contaminating enzymes. The primary components are endo-polygalacturonase and endo-pectin lyase. A brief search of the literature shows that in protoplast isolation, cellulase is often added to the digest, in addition to pectolyase.
P3026
Pectolyase from Aspergillus japonicus
lyophilized powder, ≥0.3 units/mg solid
Sinónimos:
Pectolytic enzyme
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100 MG
372,00 €
250 MG
699,00 €
1 G
1970,00 €
372,00 €
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100 MG
372,00 €
250 MG
699,00 €
1 G
1970,00 €
About This Item
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
372,00 €
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origen biológico
Aspergillus sp. (A. japonicus)
Nivel de calidad
Formulario
lyophilized powder
actividad específica
≥0.3 units/mg solid
mol peso
50 kDa
características de los productos alternativos más sostenibles
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
categoría alternativa más sostenible
, Enabling
temp. de almacenamiento
2-8°C
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Descripción general
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.[1]
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.[1]
Aplicación
Pectolyase from Aspergillus japonicus has been used:
- in the lysis of chlorella cells for protoplast generation[2]
- as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation[3]
- to induce reorganization of Arabidopsis root cap cell architecture[4]
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis[5].
Acciones bioquímicas o fisiológicas
Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Otras notas
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Definición de unidad
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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During prophase I a meiosis-specific proteinaceous tripartite structure, the synaptonemal complex (SC), forms a scaffold to connect homologous chromosomes along their lengths. This process, called synapsis, is required in most organisms to promote recombination between homologs facilitating genetic variability and
P J De Lange et al.
Annals of botany, 101(6), 881-899 (2007-12-07)
Crassula hunua and C. ruamahanga have been taxonomically controversial. Here their distinctiveness is assessed so that their taxonomic and conservation status can be clarified. Populations of these two species were analysed using morphological, chromosomal and DNA sequence data. It proved
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The Plant Journal, 4, e1102-e1102 (2014)
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Plant Physiology, 90(1), 191-196 (1989)
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Hello! I want to ask how much cellulase does the pectolyase (P3026) contains? It does not mention any contamination of other enzymes in the product description, but I want to know if there is any potential contamination involved.
1 respuesta-
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Hello, I wanted to ask if this enzyme is equivalent to pectolyase Y-23, as the activity values are expressed differently.
1 respuesta-
Yes, this product is equivalent to pectolyase Y-23.
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