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D6421

Sigma-Aldrich

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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500 ML
28,10 €

About This Item

Número MDL:
MFCD00217417
Código UNSPSC:
12352207
NACRES:
NA.75

28,10 €

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Nombre del producto

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Nivel de calidad

400

esterilidad

sterile-filtered

Formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.[1]

Aplicación

DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.
Suitable for growth of wide range of cell types in low serum conditions.
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays.[2][3][4][5] It is also used in bone marrow-derived macrophages culture.[6]

Reconstitución

Supplement with 0.365 g/L L-glutamine.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBL9056
RNBL4457
RNBL4456
RNBL1932
RNBL0502

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Yohei Ueda et al.
Scientific reports, 9(1), 8547-8547 (2019-06-14)
Growth retardation is an important side effect of glucocorticoid (GC)-based drugs, which are widely used in various preparations to treat many pediatric diseases. We investigated the therapeutic effect of exogenous CNP-53, a stable molecular form of intrinsic CNP, on a
E Zweygarth et al.
The Onderstepoort journal of veterinary research, 70(2), 165-168 (2003-09-12)
The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The cultures required supplementation of the medium with cycloheximide for
Samir A Saidi et al.
Molecular cancer, 5, 13-13 (2006-03-30)
Fenofibrate, an agonist of PPAR-alpha, in doses above 25 microM, inhibits proliferation and induces apoptosis in Ishikawa endometrial cancer cells. We show that these effects are potentiated by retinoic acid, an agonist of the retinoid-X-receptor. DNA content analysis shows that
Kouichi Hasegawa et al.
Stem cells translational medicine, 1(1), 18-28 (2012-12-01)
An optimal culture system for human pluripotent stem cells should be fully defined and free of animal components. To date, most xeno-free culture systems require human feeder cells and/or highly complicated culture media that contain activators of the fibroblast growth
Hrvoje Augustin et al.
PLoS biology, 15(9), e2001655-e2001655 (2017-09-14)
Lowered insulin/insulin-like growth factor (IGF) signaling (IIS) can extend healthy lifespan in worms, flies, and mice, but it can also have adverse effects (the "insulin paradox"). Chronic, moderately lowered IIS rescues age-related decline in neurotransmission through the Drosophila giant fiber
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