C0278

Chloroperoxidase from Caldariomyces fumago

buffered aqueous suspension, ≥3,000 units/mL

• Sinónimos: Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
• Número CAS: 9055-20-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• Número CE: 1.11.1.10 (BRENDA, IUBMB)
• MDL number: MFCD00130774
• Concentration: ≥3,000 units/mL

Propiedades

• form: buffered aqueous suspension
• Quality Segment: 200
• mol wt: 42 kDa
• concentration: ≥3,000 units/mL
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

Chloroperoxidase is a heme containing glycoprotein that is secreted from fungus. Chloroperoxidase (CPO) is a extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group.[1]

Application

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

Chloroperoxidase from Caldariomyces fumagois is useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures. It has been used to study biocatalytic oxidation in polymersome nanoreactors [2].

It has been used to study biocatalytic oxidation in polymersome nanoreactors.[2]

Biochem/physiol Actions

Chloroperoxidase (CPO) is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme and it also catalyzes peroxidase, catalase and cytochrome P450-type reactions of dehydrogenation, hydrogenperoxide (H₂O₂) decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs). PAHs are considered to be a potential health risk because of their possible carcinogenic and mutagenic activities and are widely dispersed in the environment.[3]

Physical form

Crude suspension in 0.1 M sodium phosphate, pH ~4.5

Other Notes

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H₂O₂.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)