One bottle of the lyophilized powder makes 100 mL of the cocktail. For salivary samples, a 1:500 dilution factor has been known to work well.
P2714
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder
Sinónimos:
Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
Cambiar Vistas
1 BTL
122,00 €
About This Item
Código UNSPSC:
12352200
NACRES:
NA.77
Formulario:
lyophilized powder
temp. de almacenamiento:
−20°C
Productos recomendados
Nombre del producto
Protease Inhibitor Cocktail powder, for general use, lyophilized powder
origen biológico
bovine lung
Nivel de calidad
Formulario
lyophilized powder
temp. de almacenamiento
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Descripción general
The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.
Especificidad
Inhibits serine, cysteine, and metallo-proteases.
Aplicación
Protease Inhibitor Cocktail powder has been used as a component:
- in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis[1]
- in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)[2]
- in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification[3]
- in 0.1 M NaCl solution to wash and collect skin secretions of frog[3]
Acciones bioquímicas o fisiológicas
The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Componentes
This mixture contains individual components, including:
AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM
Each component has specific inhibitory properties.
AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM
Each component has specific inhibitory properties.
Precaución
The lyophilized powder is stable for 4 years when stored at -20°C.
Cantidad
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Nota de preparación
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
Otras notas
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Irrit. 2
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Effects of phosphodiesterase 4 inhibition on alveolarization and hyperoxia toxicity in newborn rats.
Céline Méhats et al.
PloS one, 3(10), e3445-e3445 (2008-10-23)
Prolonged neonatal exposure to hyperoxia is associated with high mortality, leukocyte influx in airspaces, and impaired alveolarization. Inhibitors of type 4 phosphodiesterases are potent anti-inflammatory drugs now proposed for lung disorders. The current study was undertaken to determine the effects
Giorgio Ramadori et al.
Nature communications, 10(1), 3545-3545 (2019-08-09)
Tens of millions suffer from insulin deficiency (ID); a defect leading to severe metabolic imbalance and death. The only means for management of ID is insulin therapy; yet, this approach is sub-optimal and causes life-threatening hypoglycemia. Hence, ID represents a
Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis
Wang M, et al.
Journal of biological and chemical chronicles, 155(1), 72-76 (2010)
F Maier et al.
Osteoarthritis and cartilage, 27(9), 1382-1391 (2019-05-24)
Given the structural changes associated with the progression of Osteoarthritis (OA), we hypothesized that patterns of through-thickness, large-strain shear evolve with early-stage OA. We therefore aimed to determine whether and how patterns of shear strains change during early-stage OA to
Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Dennis SJ, et al.
Veterninary Research, 49(1), 1-6 (2018)
Artículos
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Contenido relacionado
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
-
How much PIC do I need to use in salivary samples for Western Blot?
1 respuesta-
¿Le ha resultado útil?
-
-
Hi Dose this product P2714, include EDTA? what proteases do it inhibit? Thanks, Camilla
1 respuesta-
P2714 includes EDTA at 1 mM. The components of this mixture, such as AEBSF and aprotinin, act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin among others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
¿Le ha resultado útil?
-
-
Wie löse ich das Pulver in 100 ml aqua dest???? Wie lagere ich das dann?
1 respuesta-
This product is packaged in a 10 mL serum vial with a crimp cap. Dissolve the contents of the vial in 10 mL of water or working buffer, then transfer to another container for dilution to 100 mL. Store the solution in working aliquots for at least 1 month -20 C, to avoid repeated freeze/thaw cycles. See the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/061/p2714dat-ms.pdf¿Le ha resultado útil?
-
-
I intend on taking 1mL samples of blood to give serum and test hormone levels in these samples. Should I add the solubilised protease inhibitor cocktail (p2714) directly to the blood or after centrifugation (which could be a few hours later)?
1 respuesta-
A short delay may be negligible, however, it would not be detrimental to add protease inhibitors to whole blood samples if an extended delay is anticipated. This would ensure the best results. Please see the link below to review a publication of a similar nature:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817059/¿Le ha resultado útil?
-
-
How much powder is in each bottle?
1 respuesta-
The amount in each vial is 89 mg. This material is very hygroscopic. It is recommended to prepare the entire package rather than attempt to weigh out specific amounts.
¿Le ha resultado útil?
-
-
What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
¿Le ha resultado útil?
-
-
Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?
1 respuesta-
No because it contains EDTA.
¿Le ha resultado útil?
-
-
How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?
1 respuesta-
You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use. Aliquots can be stored at -20 C for 1 month.
¿Le ha resultado útil?
-
-
How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?
1 respuesta-
One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.
¿Le ha resultado útil?
-
-
Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?
1 respuesta-
Yes, because it is water based.
¿Le ha resultado útil?
-
Filtros activos
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
