158550

Acridine Orange hemi(zinc chloride) salt

Dye content 85 %

• Sinónimos: 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
• Fórmula lineal: C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
• Número de CAS: 10127-02-3
• Peso molecular: 369.96
• Número de índice de color: 46005
• Beilstein: 3734978
• Número CE: 233-353-6
• Número MDL: MFCD00081043
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24849765
• NACRES: NA.47

Propiedades

• Formulario: powder
• Nivel de calidad: 200
• composición: Dye content, 85%
• solubilidad: H₂O: 1 mg/mL, clear, orange to very deep orange
• λmáx.: 489 nm
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
• InChI: 1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
• Clave InChI: RAHGLSRJKRXOSY-UHFFFAOYSA-L

Descripción

Aplicación

Acridine Orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs).[1][2][3]

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Acciones bioquímicas o fisiológicas

Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos.[4][5] Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test.[6]

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Warning
• Frases de peligro: H341
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Muta. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)