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Merck

E7637

Ethidium bromide

BioReagent, Molecular Biology, powder

Sinónimos:

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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Fórmula empírica (notación de Hill):
C21H20BrN3
Número CAS:
1239-45-8
Peso molecular:
394.31
UNSPSC Code:
41105322
NACRES:
NA.31
PubChem Substance ID:
24894653
EC Number:
214-984-6
Beilstein/REAXYS Number:
3642536
MDL number:
MFCD00011724

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InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

grade

Molecular Biology

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

mp

260-262 °C (dec.) (lit.)

fluorescence

λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;), λex 526 nm; λem 605 nm in aqueous buffer

suitability

suitable for gel electrophoresis

Quality Level

200

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Este artículo
E1385E8751E1510
Ethidium bromide BioReagent, Molecular Biology, powder

Sigma-Aldrich

E7637

Ethidium bromide

Ethidium bromide solution BioReagent, Molecular Biology, 500 μg/mL in H2O

Sigma-Aldrich

E1385

Ethidium bromide solution

Ethidium bromide ~95% (HPLC)

Sigma-Aldrich

E8751

Ethidium bromide

Ethidium bromide solution BioReagent, Molecular Biology, 10 mg/mL in H2O

Sigma-Aldrich

E1510

Ethidium bromide solution

grade

for molecular biology

grade

for molecular biology

grade

-

grade

for molecular biology

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

-

suitability

suitable for gel electrophoresis

fluorescence

λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm in aqueous buffer, λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;)

fluorescence

-

fluorescence

-

fluorescence

-

form

powder

form

-

form

-

form

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Frameshift mutagen which intercalates double-stranded DNA and RNA.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Preparation Note

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

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Referencia del producto
Descripción
Precios

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificados de análisis (COA)

Lot/Batch Number
MKCT4264
MKCR4318
MKCP0554
0000116938
0000094033

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Ethidium Bromide

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

Product Information Sheet - E7637

Product Information Sheet

Preguntas

1–2 de 2 Preguntas  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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