318337

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

• Sinónimos: 3,6-Bis(dimethylamino)acridine hydrochloride
• Fórmula empírica (notación de Hill): C₁₇H₁₉N₃ · HCl · xH₂O
• Número de CAS: 65-61-2
• Peso molecular: 301.81 (anhydrous basis)
• Número de índice de color: 46005
• Beilstein: 3734978
• Número CE: 200-614-0
• Número MDL: MFCD00150070
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24859123
• NACRES: NA.47

Propiedades

• Nivel de calidad: 200
• Ensayo: ≥98% (HPLC)
• Formulario: powder
• técnicas: microbe id | staining: suitable
• color: orange
• mp: 284-287 °C (lit.)
• solubilidad: H₂O: 0.1%, clear, orange to red
• λmáx.: 492 nm
• ε (coeficiente de extinción): ≥14,000 at 227-233 nm in water at 0.005 g/L; ≥35000 at 265-271 nm in water at 0.005 g/L
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2
• Clave InChI: JUAAUEXLEKAEQC-UHFFFAOYSA-N

Descripción

Aplicación

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:

• to stain intracellular acidic vesicles.[1]
• to measure lysosomal membrane permeability (LMP).[1]
• or autophagy detection.[2]

Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Acciones bioquímicas o fisiológicas

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA.[3] Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.[4]

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Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Warning
• Frases de peligro: H341
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Muta. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)