L6010

α-Lactalbumin from bovine milk

Type III, calcium depleted, ≥85% (PAGE), lyophilized powder

• Synonyme(s) : Bos d 4, Lactose synthase B protein, alpha-lactalbumin
• Numéro CAS: 9051-29-0
• UNSPSC Code: 12352202
• NACRES: NA.61
• MDL number: MFCD00162025
• Biological source: bovine milk
• Assay: ≥85% (PAGE)
• Form: lyophilized powder
• Technique(s): indirect ELISA: suitable
• Impurities: cation traces, tested
• Concentration: ≥85 % protein

Propriétés

• biological source: bovine milk
• Quality Segment: 300
• type: Type III
• assay: ≥85% (PAGE)
• form: lyophilized powder
• quality: calcium depleted
• mol wt: 14,178 Da by calculation
• concentration: ≥85 % protein
• technique(s): indirect ELISA: suitable
• impurities: cation traces, tested
• solubility: H₂O: soluble 10 mg/mL, clear to slightly hazy, colorless to faintly yellow
• cation traces: Ca: ≤0.3 mol/mol
• UniProt accession no.: P00711
• storage temp.: −20°C
• Gene Information: cow ... LALBA(281894)

Description

General description

may contain traces of (NH₄)₂SO₄ and sodium phosphateL6010

Application

α-Lactalbumin from bovine milk has been used:

• in indirect enzyme-linked immunosorbent assay (ELISA) and competitive ELISA methods
• in binding of Hsp90 and HSJ1b analysis
• to inhibit the lysozyme CAP-RAST assay

Biochem/physiol Actions

α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex.The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Angstrom resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet.

Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase.

Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp⁸⁷ or Asp⁸⁸ to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate.

Analysis Note

May contain traces of (NH₄)₂SO₄ and sodium phosphate.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, type N95 (US)