L5385
α-Lactalbumin from bovine milk
Type I, ≥85% (PAGE), lyophilized powder
Synonyme(s) :
Bos d 4, Lactose synthase B protein, alpha-lactalbumin
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About This Item
Produits recommandés
Source biologique
bovine milk
Niveau de qualité
Type
Type I
Pureté
≥85% (PAGE)
Forme
lyophilized powder
Technique(s)
cell culture | mammalian: suitable
electrophoresis: suitable
Solubilité
H2O: soluble 10 mg/mL, clear to slightly hazy, colorless to faintly yellow
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
cow ... LALBA(281894)
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Description générale
α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.
Application
α-Lactalbumin from bovine milk has been used as a supplement of basal medium for various cell cultures. It has also been used as a marker for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Actions biochimiques/physiologiques
α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet.
Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate.
Qualité
Calcium saturated. May have traces of ammonium sulfate and sodium phosphate
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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