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M2128

Sigma-Aldrich

Thiazolylblau-Tetrazoliumbromid

98%, powder

Synonym(e):

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromid, MTT, Methylthiazolyldiphenyl-tetrazoliumbromid

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100 MG
23,30 €
250 MG
39,60 €
500 MG
70,20 €
1 G
110,00 €
5 G
442,00 €
10 G
707,00 €

23,30 €

Versandbereit am09. April 2025Details

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100 MG
23,30 €
250 MG
39,60 €
500 MG
70,20 €
1 G
110,00 €
5 G
442,00 €
10 G
707,00 €

About This Item

Empirische Formel (Hill-System):
C18H16BrN5S
CAS-Nummer:
298-93-1
Molekulargewicht:
414.32
Beilstein:
4081397
EG-Nummer:
206-069-5
MDL-Nummer:
MFCD00011964
UNSPSC-Code:
12352111
PubChem Substanz-ID:
24896603
NACRES:
NA.47

23,30 €

Versandbereit am09. April 2025Details

Bulk-Bestellung anfordern

Produktbezeichnung

Thiazolylblau-Tetrazoliumbromid, 98%

Assay

98%

Form

powder

Methode(n)

titration: suitable

Farbe

dark yellow

mp (Schmelzpunkt)

195 °C (dec.) (lit.)

Löslichkeit

2-methoxyethanol: soluble 20 mg/mL
ethanol: soluble 20 mg/mL
H2O: 5 mg/mL

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

2-8°C

SMILES String

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChIKey

AZKSAVLVSZKNRD-UHFFFAOYSA-M

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Verwandte Kategorien

Anwendung

Thiazolyl Blue Tetrazolium Blue (MTT) kann zur Messung der Zellproliferation eingesetzt werden. MTT ist eine gelbliche Lösung, die sich durch mitochondriale Dehydrogenase lebender Zellen in dunkelblaues, wasserunlösliches MTT-Formazan umwandelt. Die blauen Kristalle werden mit gesäuertem Isopropanol gelöst und die Intensität wird kolorimetrisch bei 570 nm gemessen.
Thiazolyl Blue Tetrazolium Blue (MTT) kann zur Messung der Zellproliferation eingesetzt werden. MTT ist eine gelbliche Lösung, die sich durch mitochondriale Dehydrogenase lebender Zellen in dunkelblaues, wasserunlösliches MTT-Formazan umwandelt. Die blauen Kristalle werden mit gesäuertem Isopropanol gelöst und die Intensität wird kolorimetrisch bei 570 nm gemessen. MTT wird als eine histochemische/cytochemische Reagenz und zum Nachweis von NAD verwendet. ADP-verknüpfte Enzymsysteme im Gewebe können mit MTT nicht nachgewiesen werden, da das Kation durch die verwendete Cyanidfalle gebunden wird. MTT wird schnell zu Formazan reduziert, welches mit Nickel, Kupfer und Cobalt einen Chelatkomplex bildet; das Cobaltchelat wird in oxidativen Systemen verwendet.
Thiazolylblau-Tetrazoliumbromid wird für die folgenden Assays verwendet:
  • Proliferationsassay.[1][2]
  • TNF (Tumornekrosefaktor)-Bioassay.[3]

Ähnliches Produkt

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
MKCV1532
MKCR0748
MKCR7905
SHBN4138
MKCL9866

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Effect of pulse-burst electromagnetic field stimulation on osteoblast cell activities.
Chang WH, et al.
Bioelectromagnetics, 25(6), 457-465 (2004)
Xuguang Zhu et al.
Endocrine-related cancer (2019-07-05)
Anaplastic thyroid cancer (ATC) is an aggressive malignancy with limited treatment options. We explored novel treatment modalities by targeting epigenetic modifications using inhibitors of BET (e.g. BRD4) activity. We evaluated the efficacy in the treatment of ATC of a novel
Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases.
Lesslie DP, et al.
British Journal of Cancer, 94(11), 1710-1717 (2006)
Elzaan Booysen et al.
Probiotics and antimicrobial proteins, 11(3), 1009-1014 (2018-10-03)
Traditionally, methicillin-resistant Staphylococcus aureus (MRSA) is treated with vancomycin, administrated intravenously or applied directly onto infected tissue. The effect of direct (as opposed to systemic) vancomycin treatment on bone formation and remodelling is largely unknown. The minimal inhibitory concentration (MIC)
Pyrrolidine dithiocarbamate inhibits NF-kappa B mobilization and TNF production in human monocytes.
Ziegler-Heitbrock HW, et al.
Journal of Immunology, 151(12), 6986-6993 (1993)
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Protokolle

Recycling Micro-Assay of β-NAD and β-NADH

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

Fragen

1-10 von 10 Fragen  

  1. I used 0.05g in 10ml then syringe it and add 40ml. My question is how to calculate the percentage of viable cells? I used DMSO after MTT solution

    1 Antwort

    1. MTT is typically used for the semi-quantitative colorimetric measurement of cell viability. This analysis is performed in a multi-well format which screens for the viability of any given compound at any given concentration. The percentage of viable cells cannot be determined using this method. After adding a stop reagent, in this case DMSO, the formazan crystals will be dissolved, making manual counting a challenge, at best. When used in a multi-well plate format, cells are incubated, a stop solution is added, and the plate is read spectrophotometrically at 570 nM. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/305/017/m2128pis.pdf

      For additional information, see the link below to the MTT kit protocol for product TOX1, a cytotoxicity kit that utilized MTT:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      To measure cell viability as a percentage of an entire population, microscopic methods may be of interest. Reagents such as Trypan Blue allow for the clear identification of viable and non-viable cells. To review more information regarding this method, please see the link below:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/cell-quantification

      Hilfreich?

  2. What is the recommendation volume of 5 mg/ml of thiazolyl blue tetrazolium blue (in uL) per well to use for MTT assay? 

    1 Antwort

    1. Instructions for use are not provided for this product. However, there is an in vitro toxicology assay kit MTT based available. The catalog number is TOX1-1KT. In the kit, 15 mg of solid MTT is supplied. This is resuspended with 3 ml of media or a balanced salt solution without phenol red or serum. The link shared below will list the complete procedure

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      Hilfreich?

  3. What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?

    1 Antwort

    1. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).

      Hilfreich?

  4. How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?

    1 Antwort

    1. Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source:  Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.

      Hilfreich?

  5. Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 Antwort

    1. The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate.  It also corrects for dust and fingerprints that can be present.  The assay is read at 570 nm.  The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.

      Hilfreich?

  6. What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 Antwort

    1. We test the solubility of MTT in water at a concentration of 5 mg/mL.  For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.

      Hilfreich?

  7. How can I store solutions of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 Antwort

    1. Reconstituted MTT solution is stable for at least 6 months when stored frozen (-20 °C). Storage of reconstituted MTT solution at 2-8°C for more than 2 weeks may cause decomposition and yield erroneous results.

      Hilfreich?

  8. What type of multi-well plate should be used for the Thiazolyl Blue Tetrazolium Bromide (MTT) assay?

    1 Antwort

    1. It is recommended that flat bottom multi-well plates be used for the MTT assay. The use of tissue culture treated plates is only important if necessary for good cell growth.  If cells are grown in larger plates, the supernatant after cell lysis can be transferred to a 96 well plate for reading.

      Hilfreich?

  9. What is the Department of Transportation shipping information for this product?

    1 Antwort

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  10. Is the MTT assay quantitative?

    1 Antwort

    1. The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.

      Hilfreich?

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