C4428

Cathepsin G from human leukocytes

lyophilized powder, ≥60 units/mg protein (Bradford)

• CAS-Nummer: 107200-92-0
• EC-Nummer: 3.4.21.20 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130750
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human leucocytes
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥60 units/mg protein (Bradford)
• Mol-Gew.: 23-30 kDa
• UniProt-Hinterlegungsnummer: P08311
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... CTSG(1511)

Beschreibung

Allgemeine Beschreibung

Cathepsin G from human leukocytes is a neutral proteinase secreted by neutrophil granulocytes.[1] It belongs to the family of serine proteases.[2] Cathepsin G is a glycoprotein, which contains 1% carbohydrate.[1] The gene is located on human chromosome 14q12.[3]

Anwendung

Cathepsin G from human leukocytes has been used to determine protease sensitivity.[4] It has also been used for the stimulation and staining of dendritic cells and macrophage.[5]

Biochem./physiol. Wirkung

Cathepsin G from human leukocytes degrades various proteins, such as elastin, collagen, fibrinogen and immunoglobin. It may be implicated in muscle catabolism.[1] Cathepsin G is involved in the activation or inactivation of protein hormones and the inactivation of plasma proteinase inhibitors.[6] It alters the cytoskeleton, enhances the permeability of vascular endothelial cells and the chemotaxis of inflammatory cells.[2] Cathepsin G is associated with autoimmunity and cancer.[7] It exhibits chymotrypsin and trypsin like activity. Cathepsin G modulates neutrophil effector functions.[8]

Cathepsin G has pro-apoptotic activity and can activate caspases in vitro.

Einheitendefinition

One unit will release one nanomole of p-nitroaniline per second from N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide at pH 7.5 at 37 °C.

Physikalische Form

Lyophilized from 0.5 M pyridinium acetate, pH 5.3.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable