219373
Cathepsin G, humanes Neutrophil
Cathepsin G, Human Neutrophil, CAS 107200-92-0, is a purified native cathepsin G. Acts as a potent agonist of human platelet activation leading to their aggregation.
Synonym(e):
Cathepsin G, humanes Neutrophil
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About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.77
Empfohlene Produkte
Biologische Quelle
human neutrophils
Qualitätsniveau
Assay
≥95% (SDS-PAGE)
Form
lyophilized solid (Salt-free)
Spezifische Aktivität
≥2 units/mg protein
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
Methode(n)
inhibition assay: suitable
Eignung
suitable for molecular biology
Anwendung(en)
life science and biopharma
Versandbedingung
ambient
Lagertemp.
−20°C
Angaben zum Gen
human ... CTSG(1511)
Allgemeine Beschreibung
Research area: Cell Signaling
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.
Anwendung
Cathepsin G, Human Neutrophil has been used to determine the in vitro bactericidal activities of cathepsin G and for in vitro digestion of plasma/acute wound fluids.
Biochem./physiol. Wirkung
This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8. Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux. Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.
Warnhinweis
Toxizität: Standard-Handhabung (A)
Einheitendefinition
Eine Einheit ist als die Menge an Enzym definiert, die je Minute bei 25 °C und einem pH-Wert von 7,5 1,0 µmol Suc-AAPF-pNA hydrolysiert.
Angaben zur Herstellung
Aus Plasma hergestellt, das in zertifizierten Tests für HBsAg und Antikörper gegen HIV und HCV als negativ befunden wurde.
Rekonstituierung
In 50 mmol/l NaOAc und 150 mmol/l NaCl, pH-Wert 5,5, rekonstituieren.
Nach der Rekonstitution in Aliquote aufteilen und für die langfristige Lagerung einfrieren (-70 °C) oder für die kurzfristige Lagerung im Kühlschrank (4 °C) aufbewahren. Stammlösungen sind bei einer Temperatur von 4 °C bis zu 1 Woche und bei einer Temperatur von -70 °C bis zu 6 Monate haltbar.
Sonstige Hinweise
Glusa, E. und Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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