L8005

β-Lactoglobulin B from bovine milk

≥90% (PAGE)

• Synonym(e): β-LG, Bos d 5, beta-LG
• CAS-Nummer: 9066-45-9
• MDL-Nummer: MFCD00130637
• UNSPSC-Code: 12352202
• NACRES: NA.61

Eigenschaften

• Biologische Quelle: bovine milk
• Assay: ≥90% (PAGE)
• Form: powder
• Mol-Gew.: 18,276 Da by calculation
• Methode(n): HPLC: suitable; electrophoresis: suitable
• UniProt-Hinterlegungsnummer: P02754
• Lagertemp.: 2-8°C
• Angaben zum Gen: bovine ... LGB(280838)

Beschreibung

Allgemeine Beschreibung

β-Lactoglobulin B (β-LG B), a isoform of β-Lactoglobulin, is a small protein of 162 amino acids with a molecular mass of 18.2 kDa[1] and optimum pH of 5.2.[1] It is present in high level in casein and is relatively low in raw bovine milk.[1] β-LG has eight-stranded β-barrel (strands A-H) succeeded by a three-turn α-helix and a final β-strand (strand I), that forms part of the dimerization interface.[2]

Milk from dairy cows contains the protein β-lactoglobulin (BLG). It naturally occurs in a number of genetic variants, and the most prevalent bovine variants are known as BLG A and BLG B.[3]

Anwendung

β-Lactoglobulin B from bovine milk has been used as:

• a protein standard in SDS-polyacrylamide electrophoresis for quantification of milk protein fractions[4]
• in the calibration of reversed phase- high-performance liquid chromatography (HPLC) for caseins quantification[5]
• for immobilization on the biosensor surface and a calibration standards in biosensor assay[6]

β-Lactoglobulin was used in the identification of the genetic variants of κ-casein in milk by isoelectric focusing electrophoresis.[7]

Biochem./physiol. Wirkung

β-Lactoglobulin B (β-LG B) show less inhibitory effect on the Staphylaococcus sp compared to β-LG A.[8]

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)