This product is assigned a minimum activity specification of 50 units per milligram protein. At the minimum activity of 50 units, one vial would contain 20 ug of protein. The activity and protein content are lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/e8140#product-documentation
Fortfahren mit
E8140
Elastase aus Humanleukocyten
lyophilized powder, ≥50 units/mg protein (Bradford)
Synonym(e):
Lysosomal elastase
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 unit | Ab HEUTE lieferbarvonSchnelldorf | 386,00 € |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥50 units/mg protein (Bradford)
Biological source:
human leucocytes
Technischer Dienst
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Unterstützung erhaltenQuality Segment
biological source
human leucocytes
form
lyophilized powder
specific activity
≥50 units/mg protein (Bradford)
mol wt
29 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... ELA2(1991), ELANE(1991)
General description
Leukozyten Elastase ist eine 29 kDa Serinendoprotease aus der S1 Proteinase Familie. Sie existiert als einzelne 238-Aminosäuren Peptidkette mit vier Disulfidbindungen. Sie enthält ausserdem zwei oder drei N-verknüpfte Glykane von variabler Zusammensetzung, wodurch sich drei Hauptformen ergeben.
Isoelektrischer Punkt: pI = 8.77 - 9.55
Isoelektrischer Punkt: pI = 8.77 - 9.55
Elastase is a proteolytic enzyme. It is a member of the subgroup named, peptidyl peptide hydrolases. It is a major anatomic constituent of arteries. It is mainly found in the pancreas and pancreatic juice of various birds and mammals. It is also present in human serum, granulocytes and erythrocytes.
Application
Elastase from human leukocytes has been used:
- to measure serum elastase activity
- in proteolytic digestion of fibronectin and salivary glands[1]
- in neutrophil elastase (NE) activity assay
- cell-free NE digestion of E-cadherin
- scratch wound assay
- in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models
Biochem/physiol Actions
Elastase enzyme is capable of releasing soluble peptides from insoluble elastin fibers with the help of a proteolytic process. It can stimulate disintegration of the axoneme with the help of adenosine triphosphate (ATP). Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin.
Physical form
Lyophilisiert aus 0.5 M Pyridiniumacetat, pH 5.3
Other Notes
1 U setzt 1 nmol p-Nitrophenol pro Sekunde frei aus BOC-L-Alanin p-Nitrophenylester bei pH 6.5 und 37 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| Gene Information human ... ELA2(1991), ELANE(1991) | Gene Information - | Gene Information - | Gene Information - |
| specific activity ≥50 units/mg protein (Bradford) | specific activity ≥20 units/mg protein | specific activity ≥4 units/mg protein | specific activity ≥4.0 units/mg protein (biuret) |
| biological source human leucocytes | biological source - | biological source Porcine | biological source Porcine pancreas |
| form lyophilized powder | form lyophilized solid | form lyophilized powder | form lyophilized powder |
| shipped in dry ice | shipped in ambient | shipped in dry ice | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Lagerklasse
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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How much does each vial contains? please answer me using gram and not activity unit.
1 Antwort-
Hilfreich?
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Combien de temps et à quelle température peut-on stocker des aliquôts de solution l'élastase reconstituée dans le tampon pH5.5, s'il vous plaît?
1 Antwort-
This material is lyophilized from a solution containing 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl. The solution stability of this product has not been determined. However, reconstitution in water and snap-freezing in aliquots, then storing at -70°C, is recommended.
Hilfreich?
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Is this the active form of the enzyme? or does it require activation by cathepsin?
1 Antwort-
This product is not activated by cathepsin when tested for the activity of the enzyme. Please see the enzyme assay at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/506/826/e8140enz.pdfHilfreich?
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How much enzyme is contained in a single package?
1 Antwort-
As provided, each vial contains one unit of Elastase enzyme activity, defined as the amount of enzyme required to catalyze the conversion of nanomole of p-nitrophenol per second from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37 °C.
Hilfreich?
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