This product is a complex that has an estimated molecular weight of 422.5 g/mole. The solubility at 400 mM has not been tested. This product is offered in a prepared solution under product 94742 at a concentration of 200 mM. Based on the estimated molecular weight, a 0.4 M (400 mM) solution would have a concentration of 169 mg/mL. Solutions may be prepared in nuclease-free PBS containing 15% glycerol or 5% DMSO as a cryoprotectant. Solutions may then be stored in working aliquots at −20˚C. Avoid the use of EDTA in all buffer solutions as this will cause degradation of the complex. See the link below to publication that may be helpful. http://diyhpl.us/~bryan/irc/protocol-online/protocol-cache/view_cache.cgi%3FID=3486
94740
Vanadyl-Ribonukleosid-Komplexe
BioReagent, for molecular biology
Synonym(e):
Ribonucleosid-Vanadyl-Komplexe, VRC
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250 MG
119,00 €
1 G
379,00 €
119,00 €
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250 MG
119,00 €
1 G
379,00 €
About This Item
UNSPSC-Code:
12352200
NACRES:
NA.52
119,00 €
Auf LagerDetails
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Produktlinie
BioReagent
Form
powder
Eignung
in accordance for ribonuclease inhibition test
Versandbedingung
wet ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]
Anwendung
Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
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Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Inhibitors of RNases
Sambrook, J. and Russell, D.W. et al.
Molecular Cloning: A Laboratory Manual, 1, 7-7 (2001)
Tissue array for Tp53, C-myc, CCND1 gene over-expression in different tumors
Liu G Y, et al.
World Journal of Gastroenterology, 14(47), 7199-7199 (2008)
Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization
Hu F, et al.
Oncology Letters, 9(6), 2795-2798 (2015)
Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant DNA technology
Puskas R S, et al.
Biochemistry, 21(19), 4602-4608 (1982)
Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
S L Berger
Methods in enzymology, 152, 227-234 (1987-01-01)
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Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?
Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?
1 Antwort-
Hilfreich?
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How to make 200 mM from 94740-250MG vanadyl ribonucleoside complexes powder?
1 Antwort-
The method to prepare a 200mM solution of the 94740 is proprietary and involves in-process testing. However, product 94742 and R3380 are prepared 200mM solutions of Vanadyl Ribonucleoside complexes.
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