70856

RNase-A-Lösung

Convenient solution for selective degradation of RNA

• Synonym(e): RNase A Enzyme
• UNSPSC-Code: 12352202
• NACRES: NA.54

Eigenschaften

• Form: solution
• Hersteller/Markenname: Novagen^®
• Lagerbedingungen: OK to freeze
• Konzentration: 10 mg/mL
• Methode(n): DNA purification: suitable
• Versandbedingung: ambient
• Lagertemp.: 10-30°C

Beschreibung

Allgemeine Beschreibung

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA[1] purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

Anwendung

RNase A Solution has been used:

• in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)[2]
• in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays[3]
• to treat control adult and embryonic thymi cells during staining[4]
• to remove RNA from affibody–mRNA fusion clones before clone screening[5]

Biochem./physiol. Wirkung

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.[6]

Warnhinweis

Toxizität: Standard-Handhabung (A)

Rechtliche Hinweise

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2