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AMPK (α1/β1/γ3), active, His tagged human

Name: AMPK (α1/β1/γ3), active, His tagged human
Brand: SIGMA
Price: 37100 CZK

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

HAMPKb, MGC17785, MGC33776, MGC57364, PRKAA1, PRKAB1, PRKAG3

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Pack Size	SKU	Availability	Price
10 μg		Please contact Customer Service for Availability	CZK 37,100.00

About This Item

NACRES:

NA.77

UNSPSC Code:

12352200

Specific activity:

157-213 nmol/min·mg

Assay:

≥70% (SDS-PAGE)

Recombinant:

expressed in baculovirus infected Sf9 cells

CZK 37,100.00

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Properties

recombinant

expressed in baculovirus infected Sf9 cells

Quality Segment

200

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

157-213 nmol/min·mg

mol wt

A1 subunit ~68 kDa, B1 subunit ~38 kDa, G3 subunit ~51 kDa

UniProt accession no.

Q13131, Q9UGI9, Q9Y478

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKAA1(5562), PRKAB1(5564), PRKAG3(53632)

Description

Biochem/physiol Actions

AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and 3-hydroxy-3-methylglutaryl-CoA reductase. The γ subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism.

Physical form

Supplied in 50 mM Sodium Phosphate, pH 7.0, with 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

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1 of 1

This Item	A1233	A4611	SRP5004
Sigma-Aldrich A4486 AMPK (α1/β1/γ3), active, His tagged human	Sigma-Aldrich A1233 AMPK (α1/β1/γ1), active, His tagged human	Sigma-Aldrich A4611 AMPK (α1/β2/γ1), active, His tagged human	Sigma-Aldrich SRP5004 AMPK (α2/β2/γ2), active, His tagged human
Gene Information human ... PRKAA1(5562), PRKAB1(5564), PRKAG3(53632)	Gene Information human ... PRKAA1(5562), PRKAB1(5564), PRKAG1(5571)	Gene Information human ... PRKAA1(5562), PRKAB2(5565), PRKAG1(5571)	Gene Information human ... PRKAA2(5563), PRKAB2(5565), PRKAG2(51422)
assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
specific activity 157-213 nmol/min·mg	specific activity 626-848 nmol/min·mg	specific activity 297-401 nmol/min·mg	specific activity 255-345 nmol/min·mg
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
UniProt accession no. Q13131, Q9Y478, Q9UGI9	UniProt accession no. P54619, Q13131, Q9Y478	UniProt accession no. O43741, P54619, Q13131	UniProt accession no. -

pictograms

GHS08

signalword

Danger

hcodes

H360D

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

288-32-4

CAS No.

rmu

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Certificates of Analysis (COA)

Lot/Batch Number

SLBV8078

SLBH4897V

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Global Trade Item Number

SKU	GTIN
A4486-10UG	04061832847528

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