A4611

AMPK (α1/β2/γ1), active, His tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): AMPKG, MGC33776, MGC57364, MGC61468, MGC8666, PRKAA1, PRKAB2, PRKAG1
• NACRES: NA.32
• UNSPSC Code: 51111800
• Specific activity: 297-401 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 297-401 nmol/min·mg
• mol wt: A1 subunit ~68 kDa, B2 subunit ~36 kDa, G1 subunit ~40 kDa
• UniProt accession no.: O43741, P54619, Q13131
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKAA1(5562), PRKAB2(5565), PRKAG1(5571)

Description

Biochem/physiol Actions

AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and 3-hydroxy-3-methylglutaryl-CoA reductase. The β subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.

Physical form

Supplied in 50 mM Sodium Phosphate, pH 7.0, with 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable