Cell Culture Media Filters
Sterile filtration is used to protect upstream cell culture operations from contamination. Whether you need to reduce the risk of bacteria or mycoplasma contamination, our Millipore Express®, non-PFAS, cell culture media filters provide options for microorganism removal while enabling optimal cell growth and productivity and delivering the filter capacity and throughput performance you need for process economics.
Before selecting a filter, we recommend you perform a risk analysis to identify the level of bioburden control you need – a sterilizing filter or a sterilizing filter that also removes mycoplasma. Each of our filters will provide sterility assurance and give you confidence in your filtration process. In addition, our filtration portfolio is supported by the Emprove® Program which provides the documentation you need to streamline risk assessments and simplify compliance.
For help determining the right filter for your process, contact us.
STERILIZING CELL CULTURE MEDIA FILTERS
Millipore Express® SHC (Sterile, High Capacity) filters deliver extremely high filtration capacity for plugging cell culture media. The integrated 0.5 µm polyethersulfone (PES) membrane prefilter protects the 0.2 µm PES sterilizing membrane, maximizing filter capacity and improving filtration robustness.
- Millipore Express® SHC filters provide high permeability, are easy to wet and integrity test, and offer broad chemical compatibility.
- High-area capsule and cartridge formats maximize filtration efficiency without increasing footprint.
MYCOPLASMA REMOVAL FILTERS FOR CELL CULTURE MEDIA
Millipore Express® SHR filters (Sterile, High Retention) provide ≥ 7.0 log removal of mycoplasma and sterilizing-grade performance for upstream processes that need an additional level of security. The 0.1 µm mycoplasma retentive PES membrane provides high permeability and excellent microbial retention.
For more plugging cell culture media, our Millipore Express® SHRP filters contain an integrated 0.5 µm PES prefilter that protects the 0.1 µm mycoplasma retentive membrane, improving filter capacity and process robustness.
FORMATS FROM BENCH TO PRODUCTION SCALES
Our cell culture media filters offer:
- OptiScale® formats for efficient screening and scaling, and cartridge and capsule formats that are 100% integrity tested during manufacturing.
- High-area capsules and cartridge formats to maximize filtration efficiency without increasing footprint.
- Autoclavable, presterilized or gamma-compatible formats, that can be integrated into Mobius® single-use assemblies.
Approximate filtration areas of capsule and cartridge filters. Refer to product specifications for complete information and accurate filtration areas for each product line. Read our technical article for additional information on different filter formats.
QUALITY STANDARDS AND DOCUMENTATION
All Millipore Express® filters are designed, developed, and manufactured in accordance with a Quality Management System approved by an accredited registering body to an ISO 9001 Quality Systems Standard, and are supported by a certificate of quality.
All Millipore Express® SHC, Millipore Express® SHR and Millipore Express® SHRP filters are supported by the Emprove® Program which provides three types of dossiers to support different stages of development and manufacturing operations such as qualification, risk assessment, and process optimization. The dossiers consolidate comprehensive product-specific testing data, quality statements, and regulatory information in a readily available format so you can move forward with confidence.
Related Resources
- App Note: Cell Culture Media Filtration. Selection and Sizing Filters for CHO Cell Culture Processes
Explore throughput performance of Millipore Express® aseptic filters for cell culture media filtration and their impact on filter sizing.
- App Note: Cell Culture Media Filtration. Evaluating Performance of CHO Cell Cultures
Learn how to check if your media filter impacts performance. Studies confirmed PES and PVDF membrane filters did not affect cell growth and viability.
- Tech Note: Performance of High-Area Millipore Express® Cartridge Filters
High area filters can improve filtration productivity for mid- to high- plugging streams. Read how our model can help predict the benefits for different streams.
- Tech Note: Mycoplasma clearance by Millipore Express® SHR filters at pilot and production scales
Retention studies demonstrate that both pilot and production-scale filters consistently provided the expected level of mycoplasma retention over extended filtration times, affirming their performance under continuous flow processing conditions.
- App Note: Cell Culture Media Mixing and Microfiltration: Reducing Risk with Single-use Technology
This app note explains considerations for the preparation of cell culture media and focuses on the benefits of mixing and filtration using single use technologies.
- Millipore Express® Family Guide
Read this guide to review key differentiators and product specifications of Millipore Express® membrane filters.
- Tech Note: Integrity Test Troubleshooting - Beyond Rewet and Retest
Filter integrity testing doesn’t always go smoothly. Learn how to avoid costly mistakes and troubleshoot integrity test challenges.
- Brochure: Vmax™ Constant Pressure Test for sizing Aseptic Filters
This protocol describes how to use results from small scale studies to predict filtration area requirements.
- Tech Article: Aseptic Filter Formats: Selection Guide
Related Webinars
Selecting an aseptic filter for biomanufacturing depends on specific processing needs. Learn how to choose the best filter for your process.
Change management is often perceived as complex. Learn how to use a risk-based approach to expedite post-approval product changes.
This webinar explains the impact of uncontrolled bioburden, bioburden sources, and strategies to mitigate risk. Minimizing risk reduces failures and investigations.
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