XNAB2

Extract-N-Amp^™ Blood PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

• Synonym(s): Blood direct PCR kit
• NACRES: NA.55
• UNSPSC Code: 41106303

Properties

• Quality Segment: 200
• usage: sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions
• feature: dNTPs included, hotstart, Direct PCR
• technique(s): PCR: suitable
• color: colorless
• input: crude DNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, that uses an antibody based hot start and is specially formulated for amplification directly from the extract.

Genomic DNA is extracted from 10 μL of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR Mix.

The Extract-N-Amp^™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Application

Extract-N-Amp^™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples.[1][2][3][4] It has also been used for polymerase chain reaction (PCR).[5][6]

Biochem/physiol Actions

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Features and Benefits

• Efficient 8 minute prep allows greater speed and throughput
• No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
• Simple, 3 step procedure with no special equipment required
• Hot start antibody included for highly specific PCR amplification of genomic DNA
• Compatible with multiple format (single tube or 96-well plate)
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months

• Efficient 8 minute prep allows greater speed and throughput
• No need for organic extraction, centrifugation or alcohol precipitation
• Hot start antibody for highly specific PCR amplification of genomic DNA
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• L3289

  Lysis Solution for Blood

  SDS
• N9784

  Neutralization Solution for Blood

  SDS
• P8115

  Extract-N-Amp^™ PCR ReadyMix^™ for Blood

  SDS

Safety Information

• hcodes: H290,H314
• pcodes: P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Storage Class: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• pictograms: GHS05
• signalword: Danger