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P2088

Sigma-Aldrich

Peroxidase from horseradish

Peroxidase from horseradish

Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

Synonyma:

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen

Vybrat velikost

5000 UNITS
2 930,00 Kč
10000 UNITS
6 240,00 Kč

2 930,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
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Vybrat velikost

Změnit zobrazení
5000 UNITS
2 930,00 Kč
10000 UNITS
6 240,00 Kč

About This Item

Číslo CAS:
9003-99-0
Číslo enzymu podle klasifikace EK:
1.11.1.7 (BRENDA, IUBMB)
EC Number:
232-668-6
MDL number:
MFCD00071339
UNSPSC Code:
12352204
NACRES:
NA.54

2 930,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

biological source

horseradish

Quality Level

300

form

essentially salt-free, lyophilized powder

specific activity

200-300 units/mg solid (using pyrogallol)

mol wt

~44 kDa

absorbance ratio

RZ 2.6-3.4

storage temp.

2-8°C

SMILES string

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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General description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Application

Peroxidase from horseradish has been used in a study with dehydrogenative polymers (DHP) to investigate the monolignol coupling mechanism. [1] It has also been used to help develop a colorimetric method for microRNA (miRNA) detection by creating a horseradish peroxidase-mimicking DNAzyme. [2]

Biochem/physiol Actions

Horseradish peroxidase has been shown to catalyze the polymerization of various substances including acetaminophen and cardanol. [3][4]

Caution

Maintains activity at low pH and higher temperature.

Unit Definition

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Preparation Note

Stabilized by chemical protection of the primary amines.

Analysis Note

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Other Notes

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

inhibitor

Č. produktu
Popis
Stanovení ceny

S2002

Sodium azide, ReagentPlus®, ≥99.5%

substrate

Č. produktu
Popis
Stanovení ceny

D9143

o-Dianisidine, peroxidase substrate

A0156

N-(4-Aminobutyl)-N-ethylisoluminol, ≥90%

A4685

Luminol sodium salt

A1888

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)

G5502

Guaiacol, oxidation indicator

A1661

N-(6-Aminohexyl)-N-ethylisoluminol

A3537

5-Aminosalicylic acid, ≥99%

A4382

4-Aminoantipyrine, reagent grade

A8264

4-Aminophthalhydrazide

A5754

3-Amino-9-ethylcarbazole, ≥95% (HPLC), powder

A8511

Luminol, ≥97% (HPLC)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Osvědčení o analýze (COA)

Lot/Batch Number
0000320424
0000320424
0000287739
0000287739
SLCQ2073

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Keehoon Won et al.
Biomacromolecules, 5(1), 1-4 (2004-01-13)
Horseradish peroxidase-catalyzed polymerization of cardanol in aqueous organic solvent was investigated in the presence of a redox mediator. Cardanol is a phenol derivative from a renewable resource mainly having a C15 unsaturated hydrocarbon chain with mostly 1-3 double bonds at
D W Potter et al.
Molecular pharmacology, 29(2), 155-162 (1986-02-01)
Horseradish peroxidase catalyzed the polymerization of acetaminophen. Addition of reduced glutathione (GSH) to reaction mixtures resulted in decreased polymerization and formation of minor amounts of GSH-acetaminophen conjugates. The conjugates were identified as 3-(glutathion-S-yl)acetaminophen and 3-(glutathion-S-yl)diacetaminophen. Horseradish peroxidase also catalyzed polymerization
Yanqin Wen et al.
Analytical chemistry, 84(18), 7664-7669 (2012-08-30)
We present a highly sensitive colorimetric method for microRNA (miRNA) detection. This method is based on a rolling-circle amplification (RCA) DNA machine, which integrates RCA, nicking enzyme signal amplification and DNAzyme signal amplification. The DNA machine is triggered by the
Sun-Joo Moon et al.
Phytochemistry, 82, 15-21 (2012-08-14)
In this study, dehydrogenative polymers (DHP) were synthesized in vitro through dehydrogenative polymerization using different ratios of coniferyl alcohol (CA) and sinapyl alcohol (SA) (10:0, 8:2, 6:4, 2:8, 0:10), in order to investigate the monolignol coupling mechanism in the presence
Kristina Kühn et al.
The Plant cell, 21(9), 2762-2779 (2009-09-29)
Transcription of mitochondrial genes in animals, fungi, and plants relies on the activity of T3/T7 phage-type RNA polymerases. Two such enzymes, RPOTm and RPOTmp, are present in the mitochondria of eudicotyledonous plants; RPOTmp is additionally found in plastids. We have
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Sortimentní položky

Horseradish Peroxidase (HRP) Enzymes

Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.

Protokoly

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

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