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horseradish
assay
90% (HPLC)
form
lyophilized (salt-free)
specific activity
~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2)
packaging
pkg of 100 mg
manufacturer/tradename
Roche
concentration
10 g/L
technique(s)
activity assay: suitable, protein detection: suitable
color
red to brown
optimum pH
6.0-6.5
solubility
water: soluble at 68 °F
suitability
suitable for molecular biology
UniProt accession no.
application(s)
detection
life science and biopharma
foreign activity
ATPase <0.00100%, none detected, AcP <0.00100%, Catalase <0.700%
shipped in
dry ice
storage temp.
−20°C (−15°C to −25°C)
General description
Donor: hydrogen-peroxide oxidoreductase
Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.
Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.
Preparation Note
Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H2O2) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H2O2, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H2O2)
Amino groups: 2 - 3moles/mole enzyme
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H2O2) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H2O2, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H2O2)
Amino groups: 2 - 3moles/mole enzyme
Carbohydrates: 12.0 - 14.5% (w/w)
Isoenzymes: ≥90% isoenzyme C (HPLC)
Purity: single peak (HPLC)
Purity number: 3.0 - 3.5 (A403/A275)
Isoenzymes: ≥90% isoenzyme C (HPLC)
Purity: single peak (HPLC)
Purity number: 3.0 - 3.5 (A403/A275)
Keep container tightly closed in a dry and well-ventilated place.
Other Notes
Blocking substances
Preparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH2-groups/mol does not change.
POD
POD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca2+.
POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes).
Carbohydrate Content
12 to 14.5 mg/100 mg Iyophilizate
Free Amino Groups
Free amino groups: 2 to 3 mol/mol enzyme
Preparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH2-groups/mol does not change.
POD
POD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca2+.
POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes).
Carbohydrate Content
12 to 14.5 mg/100 mg Iyophilizate
Free Amino Groups
Free amino groups: 2 to 3 mol/mol enzyme
For life science research only. Not for use in diagnostic procedures.
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Výstražné slovo
Danger
Kódy nebezpečí
Bezpečnostní pokyny
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Skladovací třída
11 - Combustible Solids
Co se děje?
WGK 1
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
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