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Key Documents

MAK338

Sigma-Aldrich

Fatty Acid Extraction Kit, High Standard

greener alternative

sufficient for 40 extractions

Synonyma:

Lipid Extraction Kit

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 40 extractions

greener alternative product characteristics

Waste Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

input

plasma
food(s) (oil)
blood
tissue extract(s)
food(s)
serum

application(s)

cosmetics
food and beverages
sample preparation

greener alternative category

relevant disease(s)

cancer

storage temp.

room temp

General description

Lipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids.

When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 Fatty Acid Extraction Kit. This kit produces stronger signals. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 Fatty Acid Extraction Kit. If in doubt about which kit to use, use MAK338. The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.

The Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in the kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.

Application

The Fatty Acid Extraction Kit may be used for:
  • Lipid Analysis
  • Lipidomics Research

Features and Benefits

Simple and Efficient: Simplify the lipid extraction process using the premade extraction solvent and extraction syringe to rapidly elute the fatty acids for analysis, saving you time and effort.

Suitability

This kit is suitable for the extraction of lipids from a variety of samples such as whole blood, serum, biological tissue, food samples, and oil.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

related product

Č. produktu
Popis
Stanovení ceny

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 1 - STOT SE 3

target_organs

Central nervous system, Eyes, Liver,Kidney

Storage Class

3 - Flammable liquids

flash_point_f

95.0 °F

flash_point_c

35 °C


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