I1146

R³ IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyma: Human IGF-1, IGF-1 Protein, Recombinant IGF-1
• UNSPSC Code: 51111800
• NACRES: NA.77

Vlastnosti

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥85% (HPLC)
• form: lyophilized powder
• potency: ≤10 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 7,676 Da
• packaging: pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P05019
• storage temp.: 2-8°C
• Gene Information: human ... IGF1(3479)

Popis

Application

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Biochem/physiol Actions

Proliferation of chinese hamster ovary CHO cells.

Other Notes

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Preparation Note

Prepared from 0.1 M acetic acid

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)