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Key Documents

H4502

Sigma-Aldrich

Hexokinase from Saccharomyces cerevisiae

Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

Synonyma:

Hexokinase D, Hexokinasetype IV, ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

Číslo CAS:
Číslo enzymu podle klasifikace EK:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Saccharomyces cerevisiae

Quality Level

type

Type F-300

form

lyophilized powder

specific activity

≥130 units/mg protein (biuret)

mol wt

110 kDa

purified by

crystallization

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

color

white

anion traces

sulfate (SO42-): free

UniProt accession no.

foreign activity

ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%

storage temp.

−20°C

Gene Information

bakers yeast ... HXK1(850614) , HXK2(852639)

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General description

Research area: Cell Signaling

The hexokinase 1 (HXK1) gene is mapped to the Saccharomyces cerevisiae chromosome VI. HXK1 is ubiquitously expressed in mammalian tissues and is abundant in the brain, erythrocytes, lymphocytes, and fibroblasts.

Application

Hexokinase has been used:
  • for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutina
  • to treat ADP (adenosine diphosphate) solution along with D-glucose to remove the contaminating ATP (adenosine triphosphate)
  • in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.5) for ADP-[13CU]glucose synthesis

Biochem/physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Hexokinase catalyzes the rate-limiting first step of glycolysis, which involves the phosphorylation of glucose.

Quality

A mixture of isoenzymes.

Unit Definition

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing approx. 15% sodium citrate

Reconstitution

Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.

related product

Č. produktu
Popis
Stanovení ceny

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Osvědčení o analýze (COA)

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Protokoly

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

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