GE28-4083-47
Ab Spintrap™
Cytiva 28-4083-47
About This Item
Doporučené produkty
ligand
recombinant protein G lacking albumin-binding region
packaging
pkg of 50 × 100 μL
manufacturer/tradename
Cytiva 28-4083-47
storage condition
(20% Ethanol)
bed volume
100 μL
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-9
working range
3-9
capacity
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(IgG/column)
storage temp.
2-8°C
Související kategorie
General description
Application
The spin columns are also suitable for use in antibody screening experiments.
Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.
Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.
For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.
Features and Benefits
- Rapid and convenient small-scale purification of IgG.
- Protein G Sepharose™ High Performance in prepacked spin columns.
- No filtration or centrifugation of different crude samples necessary for antibody purification.
- Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
- Elution conditions formatted for both LC-MS and electrophoresis analysis.
- Elution conditions formatted for both LC-MS and electrophoresis analysis.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Sortimentní položky
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Protokoly
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
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