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Key Documents

GE17-0921-02

HiTrap® IMAC Fast Flow

Cytiva 17-0921-02, pack of 5 × 1

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

packaging

pack of 5 × 1

manufacturer/tradename

Cytiva 17-0921-02

parameter

42 psi

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14(Medium uncharged with metal ions.)

working range

3-12

capacity

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

suitability

suitable for bioprocess medium

General description

HiTrap® IMAC FF is prepacked with IMAC Sepharose 6 Fast Flow. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Application

IMAC Sepharose FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.


IMAC Sepharose 6 Fast FLow consists of 90 ?m beads of highly cross-linked Agarose to which a chelating group has been covalently coupled. This chelating group can be charged with suitable metal ions, specific for your target Protein of interest. The bead size of IMAC Sepharose 6 Fast FLow enables high flow properties, making the medium an excellent choice for scaling up purification at laboratory scale and purification of histidine-tagged Proteins at process scale. Low metal ion leakage means that the activity of the purified Protein is retained and the risk of precipitation reduced, which results in increased purity, activity, and yield of the target Protein. Leakage of metal ions into the eluted target Protein pool from IMAC Sepharose 6 Fast FLow is generally Low under normal conditions.


IMAC Sepharose 6 Fast FLow is highly stable and compatible with a wide range of common additives. This helps maintain biological activity and increase product yield, while at the same time greatly expanding the range of suitable operating conditions.


The medium is available prepacked in HiTrap® HiScreen and HiPrep column formats for convenient purification of histidine-tagged Proteins at laboratory scale. Initial purification using a 1 mL HiTrap® IMAC FF column can be scaled up further with the 5 mL column. Purification can be scaled up even more using 20 mL HiPrep IMAC FF 16/10 columns. Prepacked HiScreen IMAC FF with a 10 cm bed height is the format of choice for method optimization and process development.

Features and Benefits

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
  • Conveniently charge with your metal of choice.
  • Optimal bead size for scaling up.
  • High binding capacity.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiPrep is a trademark of Cytiva
HiScreen is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Related product

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Osvědčení o analýze (COA)

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Sortimentní položky

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Zobrazit všechny

Protokoly

This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.