GE17-0921-07
IMAC Sepharose™ 6 Fast Flow
Cytiva 17-0921-07, pack of 25 mL
About This Item
Doporučené produkty
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-0921-07
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
working range
3-12
capacity
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
suitability
suitable for bioprocess medium
General description
Application
IMAC Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose™ 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.
The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.
IMAC Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support
Features and Benefits
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in the convenient and time-saving prepacked HiTrap® and HiPrep™ formats.
Storage and Stability
Analysis Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Sortimentní položky
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Tato stránka ukazuje, jak purifikovat nebo odstraňovat proteiny a peptidy s exponovanými aminokyselinami pomocí chelatační sepharosy High Performance, chelatační sepharosy Fast Flow, Capto Chelating od společnosti Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
Protokoly
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Tato stránka ukazuje, jak purifikovat rekombinantní proteiny značené histidinem z neklonovaného buněčného lyzátu pomocí HisTrap FF crude od společnosti Cytiva.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis.