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GE17-5319-01

HisTrap™ Fast Flow

Cytiva 17-5319-01, pack of 5 × 1 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5319-01

parameter

<20 mL/min flow rate
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

~40 mg binding capacity(histidine-tagged protein)

suitability

suitable for bioprocess medium

General description

HisTrap™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Features and Benefits

  • High binding capacity, approx. 40 mg/mL medium.
  • Negligible leakage of Ni2+.
  • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Environment
GHS09

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

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Lot/Batch Number

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Sortimentní položky

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This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

Protokoly

Cell Disruption and Membrane Preparation

This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.

Affinity Chromatography in a Purification Strategy (CIPP)

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using Cytiva products.

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

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