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G1270

Sigma-Aldrich

L-Glutamine Synthetase from Escherichia coli

lyophilized powder, 400-2,000 units/mg protein

Synonyma:

L-Glutamate:ammonia ligase (ADP-forming)

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About This Item

Číslo CAS:
9023-70-5
Číslo enzymu podle klasifikace EK:
6.3.1.2 (BRENDA, IUBMB)
MDL number:
MFCD00131463
UNSPSC Code:
12352204
NACRES:
NA.26

form

lyophilized powder

Quality Level

200

specific activity

400-2,000 units/mg protein

purified by

affinity chromatography

contains

dithioerythritol as preservative

composition

Protein, ~5% Lowry

solubility

H2O: soluble 0.95-1.05 mg/mL, clear to hazy

UniProt accession no.

P0A9C5

foreign activity

ATPase <0.2%

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... glnA(948370)

General description

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

Application

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

Biochem/physiol Actions

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.
Degradative enzyme for glutamic acid

Unit Definition

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

Physical form

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Osvědčení o analýze (COA)

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Navštívit knihovnu dokumentů

Reversible Adenylylation of Glutamine Synthetase Is Dynamically Counterbalanced during Steady-State
Okano H, et al.
Journal of molecular biology, 404(1), 522-536 (2010)
Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation
Jin DY, et al.
The Journal of Biological Chemistry, 272(49), 30952-30961 (1997)
Ammonia acquisition in enteric bacteria: physiological role of the ammonium/methylammonium transport B (AmtB) protein
Soupene E, et al.
Proceedings of the National Academy of Sciences of the USA, 95(12), 7030-7034 (1998)
Structural Analysis of Glutamine Synthetase from Helicobacter pylori
Joo HK, et al.
Scientific Reports, 8(1), 11657-11657 (2018)
Kamal Krishna Singh et al.
Plant cell reports, 32(2), 183-193 (2012-10-17)
KEY MESSAGE : The regulation of GS isoforms by WD was organ specific. Two GS isoforms i.e. OsGS1;1 and OsGS2 were differentially regulated in IR-64 (drought-sensitive) and Khitish (drought-tolerant) cultivars of rice. Water deficit (WD) has adverse effect on rice
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