Various solvents, including ethanol, are reported to inhibit forskolin-mediated activation of adenylate cyclase. Dimethyl sulfoxide (DMSO) is the recommended solvent; at a final concentration of 5% or less, little if any inhibition of adenylate cyclase activation will occur. A 50 mM stock solution of forskolin can be prepared by solubilizing 10 mg of forskolin in 488 µL of fresh, high purity, and anhydrous DMSO. After reconstitution, aliquot and freeze at -20°C. The 50 mM stock solution can be diluted 1:100 in DMSO to achieve a 500 µM working stock solution.
F3917
Forskolin
For use in molecular biology applications
Synonyma:
7β-Acetoxy-8,13-epoxy-1α,6β,9α-trihydroxylabd-14-en-11-one, Coleonol, Colforsin
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Vybrat velikost
10 MG
6 850,00 Kč
25 MG
21 100,00 Kč
6 850,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Vybrat velikost
Změnit zobrazení
10 MG
6 850,00 Kč
25 MG
21 100,00 Kč
About This Item
Empirický vzorec (Hillův zápis):
C22H34O7
Číslo CAS:
Molekulová hmotnost:
410.50
Beilstein/REAXYS Number:
1692716
EC Number:
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.52
6 850,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Doporučené produkty
biological source
Coleus forskohlii
Quality Level
description
for activation of T cells
product line
BioReagent
assay
≥98% (HPLC)
form
powder
mol wt
410.50 g/mol
color
white to off-white
solubility
ethanol: soluble
suitability
suitable for inhibits interleukin 2 (IL-2) production in Jurkat cells
storage temp.
room temp
SMILES string
CC(=O)O[C@H]1[C@@H](O)[C@H]2C(C)(C)CC[C@H](O)[C@]2(C)[C@@]3(O)C(=O)C[C@@](C)(O[C@]13C)C=C
InChI
1S/C22H34O7/c1-8-19(5)11-14(25)22(27)20(6)13(24)9-10-18(3,4)16(20)15(26)17(28-12(2)23)21(22,7)29-19/h8,13,15-17,24,26-27H,1,9-11H2,2-7H3/t13-,15-,16-,17-,19-,20-,21+,22-/m0/s1
InChI key
OHCQJHSOBUTRHG-KGGHGJDLSA-N
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General description
Forskolin is a cell permeable diterpenoid isolated from Coleus forskohlii.
Biochem/physiol Actions
Cell-permeable diterpenoid that possesses anti-hypertensive, positive inotropic, and adenylyl cyclase activating properties. Many of its biological effects are due to its activation of adenylyl cyclase and the resulting increase in intracellular cAMP concentration. Forskolin effects calcium currents and inhibits MAP kinase.
related product
Č. produktu
Popis
Stanovení ceny
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Osvědčení o analýze (COA)
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Protokoly
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
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How can i prepare 500uM Forskolin (10mg) and where can the prepared solution be stored?
1 answer-
Helpful?
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What is the shelf life of the solid form of forskolin?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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How should I store a DMSO or ethanol solution of Product F3917, Forskolin?
1 answer-
Forskolin solutions at 5 mg/mL in either ethanol or DMSO have been found to be stable for at least 6 months at room temperature, as determined by HPLC analysis.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I make an aqueous solution of Product F3917, Forskolin?
1 answer-
Forskolin is soluble in water (with 2% ethanol) up to 0.2 mM by first dissolving in ethanol at 5 mg/mL and performing subsequent dilutions in water. Similar results were obtained with DMSO.
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Will residual solvents inhibit forskolin activation of adenylate cyclase?
1 answer-
Various solvents, including ethanol, inhibit the forskolin activation of adenylate cyclase. DMSO is recommended for preparing forskolin solutions, because at concentrations of 5% or less of DMSO, there is little if any inhibition of forskolin activation.
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