AB1 cell line from mouse lung

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 10092305, mouse lung. Fibroblast-like, but demonstrates typical epithelial cobblestone morphology

• UNSPSC Code: 41106514

Vlastnosti

• biological source: mouse lung
• description: Mouse malignant mesothelioma
• packaging: tube of 5 μg 10092305-DNA-5UG; pkg of vial of cells 10092305-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast-like but shows a typical epithelial "cobblestone"
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Mouse malignant mesothelioma

Cell Line Description

Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0144

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Culture Medium

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS

Subculture Routine

Optimal split ratio 1:8 (seeding density 1.2 x104 cells/cm²).Cells incubated at 37oC with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Other Notes

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake, University of Western Australia, Australia