L929 Cell Line from mouse

85011425, mouse adipose tissue, Fibroblast

• Synonyma: Earles′s Cells, L Cells, NCTC clone 929 Cells
• UNSPSC Code: 41106514

Vlastnosti

• product name: L929 Cell Line from mouse, mouse C3H/An connective tisue, 85011425
• biological source: mouse adipose tissue
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Mouse C3H/An connective tissue

Cell Line Description

Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.

Application

Virus studies: PRV, VSV (Indiana), HSV

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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