AE17 cell line from mouse lung

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 10092310, mouse lung, Cells are epithelial-like

• Synonyma: AE17 Cells
• UNSPSC Code: 41106514
• Biological source: mouse lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Cells are epithelial-like
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: mouse lung
• description: Mouse malignant mesothelioma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 10092310-DNA-5UG, pkg of vial of cells 10092310-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Cells are epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Biochem/physiol Actions

Mice (C57Black/6J strain female 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0156

Mouse malignant mesothelioma

Preparation Note

Optimal split ratio 1:16 - 1:32 (seeding density 0.6 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

Other Notes

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.