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30674

Sigma-Aldrich

Atto 700

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

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About This Item

MDL number:
MFCD06798563
UNSPSC Code:
41116100
NACRES:
NA.32

product line

BioReagent

assay

≥90.0% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
700 nm

fluorescence

λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 692 nm ± 3 nm)

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 700 is a new label with high molecular absorption (1.2 x 105) and quantum yield (0.25) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 700 is characterized by high photostability and thermal stability. It is also excellently soluble in water. Due to an insignificant triplet formation rate, it is well suited for single molecule detection applications.

Application

Atto 700 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, excellent thermal and photo-stability, very good water solubility and very little triplet formation. Atto 700 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.
Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Osvědčení o analýze (COA)

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Multicolour single molecule imaging in cells with near infra-red dyes.
Tynan, C.J., et al.
PLoS ONE, 4, e36265-e36265 (2012)
Jan Vogelsang et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 486-496 (2009-04-02)
The role and interplay of triplet states and radical ion states in single-molecule fluorescence spectroscopy has recently been elaborated providing us with new insights into the photophysics and photobleaching pathways of fluorescent dyes. Adjustment of fluorophore redox properties in combination
Patricia Haus et al.
Journal of biomolecular screening, 16(10), 1206-1216 (2011-10-27)
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Daniel Riester et al.
Bioorganic & medicinal chemistry letters, 19(13), 3651-3656 (2009-05-22)
Histone deacetylases reside among the most important and novel target classes in oncology. Selective lead structures are intensively developed to improve efficacy and reduce adverse effects. The common assays used so far to identify new lead structures suffer from many
Judith E Berlier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)
Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were
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