FIBRP-RO

Fibronectin (pure)

from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

• Synonyma: Fibronectin
• MDL number: MFCD00131062
• UNSPSC Code: 12352202

Vlastnosti

• biological source: human plasma
• Quality Level: 100
• sterility: non-sterile; 0.2 μm filtered
• assay: >95% (SDS-PAGE)
• form: lyophilized (clear, colorless solution after reconstitution)
• mol wt: 440 kDa
• packaging: pkg of 1 mg (11051407001); pkg of 5 mg (11080938001)
• manufacturer/tradename: Roche
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P02751
• storage temp.: 2-8°C
• Gene Information: human ... FN1(2335)

Popis

General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Specificity

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

Sequence

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Physical form

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Preparation Note

Working concentration: 5μg/cm² for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash